Over the last decade, novel technologies for noninvasive imaging of skin have been investigated, including optical coherence tomography, confocal reflectance microscopy, confocal fluorescence microscopy, and spectroscopy. Noninvasive imaging offers exciting applications including screening and diagnosis in vivo, assessing tumor characteristics, mapping surgical margins, and monitoring treatments. Confocal microscopy provides high-resolution images in skin with sharp nuclear and cellular detail, and with demonstrated sensitivity and specificity for detecting malignancies.
Our group has demonstrated that basal cell carcinomas (BCCs) are easily visualized with high resolution in real time on excised Mohs surgery specimens, with contrast agents such as acetowhitening in reflectance and acridine orange in fluorescence confocal imaging without routine frozen section processing.
More recently, newly developed microscopes offer a significantly faster approach for imaging BCCs, offering the possibility of real-time detection of tumor margins at the bedside to potentially guide Mohs surgery. The goal of this project is to test high-speed confocal microscopy for the detection of basal cell and squamous cell carcinomas in excised tissue from Mohs surgery.
Preferred Project Dates
Students are expected to be in attendance all eight weeks, start and end dates inclusive.