Major Research Areas

Selected Publications

Cheng C, Shuman S. (2000) DNA strand transfer catalyzed by vaccinia topoisomerase: ligation of DNAs containing a 3' mononucleotide overhang. Nucleic Acids Res. 2000;28:1893-1898.

Wittschieben J, Shuman S. (1997) Mechanism of DNA transesterification by vaccinia topoisomerase: catalytic contributions of essential residues Arg-130, Gly-132, Tyr-136, and Lys-167. Nucleic Acids Res. 1997;25:3001-3008.

Wang LK, Wittschieben J, Shuman S. (1997) Mutational analysis of 26 residues of vaccinia DNA topoisomerase identifies Ser-204 as important for DNA binding and cleavage. Biochemistry. 1997;36:7944-7950.

Sekiguchi J, Shuman S. (1997) Site-specific ribonuclease activity of eukaryotic topoisomerase I. Mol Cell. 1997;1:89-97.

Wittschieben J, Petersen BO, Shuman S. (1998) Replacement of the active site tyrosine of vaccinia DNA topoisomerase by glutamate, cysteine, or histidine converts the enzyme into a site-specific endonuclease. Nucleic Acids Res. 1998;26:490-496.

Cheng C, Kussie P, Pavletich N, Shuman S. (1998) Conservation of structure and mechanism between eukaryotic topoisomerase I and site-specific recombinases. Cell. 1998;92:841-850.

Shuman S. (1998) Polynucleotide ligase activity of eukaryotic topoisomerase I. Mol. 1998;1:741-748.

Cheng C, Shuman S. (1998) A catalytic domain of eukaryotic DNA topoisomerase I. J Biol Chem. 1998;273:11589-11595.

Krogh BO, Cheng C, Burgin A Jr, Shuman S. (1999) Melanoplus sanguinipes entomopoxvirus DNA topoisomerase: site-specific DNA transesterification and effects of 5'-bridging phosphorothiolates. Virology. 1999;264:441-451.

Krogh BO, Shuman S. (2002) Proton relay mechanism of general acid catalysis by DNA topoisomerase IB. J Biol Chem. 2002;27:5711-5714.

Krogh BO, Shuman S. (2000) DNA strand transfer catalyzed by vaccinia topoisomerase: peroxidolysis and hydroxylaminolysis of the covalent protein-DNA intermediate. Biochemistry. 2000;39:6422-6432.

Sekiguchi J, Cheng C, Shuman S. (1997) Kinetic analysis of DNA and RNA strand transfer reactions catalyzed by vaccinia topoisomerase. J Biol Chem. 1997;272:15721-15728.

Stivers JT, Jagadeesh GJ, Nawrot B, Stec WJ, Shuman S. (2000) Stereochemical outcome and kinetic effects of Rp and Sp phosphorothioate substitutions at the cleavage site of vaccinia type I DNA topoisomerase. Biochemistry. 2000;39:5561-5572.

Klemm M, Cheng C, Cassell G, Shuman S, Segall AM. (2000) Peptide inhibitors of DNA cleavage by tyrosine recombinases and topoisomerases. J Mol Biol. 2000;299:1203-1216.

Krogh BO, Shuman S. (2000) Catalytic mechanism of DNA topoisomerase IB. Mol Cell. 2000;5:1035-1041.

Sekiguchi J, Cheng C, Shuman S. (2000) Resolution of a Holliday junction by vaccinia topoisomerase requires a spacer DNA segment 3' of the CCCTTØ cleavage sites. Nucleic Acids Res. 2000;28:2658-2663.

Woodfield G, Cheng C, Shuman S, Burgin AB. (2000) Vaccinia topoisomerase and Cre recombinase catalyze direct ligation of activated DNA substrates containing a 3'-para-nitrophenyl phosphate ester. Nucleic Acids Res. 2000;28:3323-3331.

Cheng C, Shuman S. (2000) A recombinogenic flap ligation pathway for intrinsic repair of topoisomerase IB-induced double strand breaks. Mol Cell Biol. 2000;20:8059-8068.

Krogh BO, Claeboe CD, Hecht SM, Shuman S. (2001) Effect of 2'-5' phosphodiesters on DNA transesterification by vaccinia topoisomerase. J Biol Chem. 2001;276:20907-20912.

Krogh BO, Shuman S. (2001) Vaccinia topoisomerase mutants illuminate conformational changes during closure of the protein clamp and assembly of a functional active site. J Biol Chem. 2001;276:36091-36099.

Krogh BO, Shuman S. (2002) A poxvirus-like type IB topoisomerase family in bacteria. Proc Natl Acad Sci USA. 2002;99:1853-1858.

Cheng C, Shuman S. (1999) Site-specific transesterification by vaccinia topoisomerase: role of specific phosphates and nucleosides. Biochemistry. 1999;38:16599-165612.

Sekiguchi J, Stivers JT, Mildvan AS, Shuman S. (1996) Mechanism of inhibition of vaccinia DNA topoisomerase by novobiocin and coumermycin. J Biol Chem. 1996;271:2313-2322.

Shuman S. (1992) DNA strand transfer reactions catalyzed by vaccinia topoisomerase I. J Biol Chem. 1992;267:8620-8627.

Shuman S. (1992) Two classes of DNA end-joining reactions catalyzed by vaccinia topoisomerase I. J Biol Chem. 1992;267:16755-16758.

Stivers JT, Shuman S, Mildvan AS. (1994) Vaccinia DNA topoisomerase I: single-turnover and steady-state kinetic analysis of the DNA strand cleavage and ligation reactions. Biochemistry. 1994;33:327-339.

Wittschieben J, Shuman S. (1994) Mutational analysis of vaccinia virus DNA topoisomerase defines amino acid residues essential for covalent catalysis. J Biol Chem. 1994;269:29978-29983.

Sekiguchi J, Shuman S. (1994) Requirements for noncovalent binding of vaccinia topoisomerase I to duplex DNA. Nucleic Acids Res. 1994;22:5360-5365.

Sekiguchi J, Shuman S. (1994) Stimulation of vaccinia DNA topoisomerase by nucleoside triphosphates. J Biol Chem. 1994;269:29760-29764.

Stivers JT, Shuman S, Mildvan AS. (1994) Vaccinia DNA topoisomerase I: kinetic evidence for general acid-base catalysis and a conformational step. Biochemistry. 1994;33:15449-15458.

Sekiguchi J, Shuman S. (1994) Vaccinia topoisomerase binds circumferentially to DNA. J Biol Chem. 1994;269:31731-31734.

Shuman S. (1994) Novel approach to molecular cloning and polynucleotide synthesis using vaccinia DNA topoisomerase. J Biol Chem. 1994;269:32678-32684.

Shuman S, Bear DG, Sekiguchi J. (1997) Intramolecular synapsis of duplex DNA by vaccinia topoisomerase. EMBO J. 1997;16:6584-6589.

Petersen BO, Wittschieben J, Shuman S. (1996) Mutations within a conserved region of vaccinia topoisomerase affect the cleavage-religation equilibrium. J Mol Biol. 1996;263:181-195.

Sekiguchi J, Shuman S. (1997) Mutational analysis of vaccinia virus topoisomerase identifies residues involved in DNA binding. Nucleic Acids Res. 1997;25:3649-3656.

Sekiguchi J, Seeman NC, Shuman S. (1996) Resolution of Holliday junctions by eukaryotic DNA topoisomerase I. Proc Natl Acad Sci USA. 1996;93:785-789.

Sekiguchi J, Shuman S. (1996) Identification of contacts between topoisomerase I and its target DNA by site-specific photocrosslinking. EMBO J. 1996;15:3448-3457.

Sekiguchi J, Shuman S. (1996) Covalent DNA binding by vaccinia topoisomerase results in unpairing of the thymine base 5' of the scissile bond. J Biol Chem. 1996;271:19436-19442.

Petersen BO, Hall RL, Moyer RW, Shuman S. (1997) Characterization of a DNA topoisomerase encoded by Amsacta moorei entomopoxvirus. Virology. 1997;230:197-206.

Petersen BO, Shuman S. (1997) Histidine-265 is important for covalent catalysis by vaccinia topoisomerase and is conserved in all eukaryotic type I enzymes. J Biol Chem. 1997;272:3891-3896.

Petersen BO, Shuman S. (1997) DNA strand transfer reactions catalyzed by vaccinia topoisomerase: hydrolysis and glycerololysis of the covalent protein-DNA intermediate. Nucleic Acids Res. 1997;25:2091-2097.

Wang LK, Shuman S. (1997) Deletions at the carboxyl terminus of vaccinia DNA topoisomerase affect DNA binding and enhance distributivity in DNA relaxation. Biochemistry. 1997;36:3909-3916.

Cheng C, Wang LK, Sekiguchi J, Shuman S. (1997) Mutational analysis of 39 residues of vaccinia DNA topoisomerase identifies Lys-220, Arg-223, and Asn-228 as important for covalent catalysis. J Biol Chem. 1997;272:8263-8269.

Shuman S. (1991) Recombination mediated by vaccinia DNA topoisomerase I in Escherichia coli is sequence specific. Proc Natl Acad Sci USA. 1991;88:10104-10108.

Sekiguchi J, Shuman S. (1995) Proteolytic footprinting of vaccinia topoisomerase I bound to DNA. J Biol Chem. 1995;270:11636-11645.

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