Major Research Areas

Selected Publications

Pei Y, Hausmann S, Ho CK, Schwer B, Shuman S. 1999 Distinct roles for CTD Ser2 and Ser5 phosphorylation in the recruitment and allosteric activation of mammalian capping enzyme. J Biol Chem. 2001;276:28075-28082.

Schwer B, Saha N, Mao X, Chen HW, Shuman S. 2000 Structure-function analysis of yeast mRNA cap methyltransferase and high-copy suppression of conditional mutants by AdoMet synthase and the ubiquitin conjugating enzyme Cdc34p. Genetics. 2000;155;1561-1576.

Shuman S. 1997 Origins of mRNA identity: capping enzymes bind to the phosphorylated C-terminal domain of RNA polymerase II. Proc Natl Acad Sci USA. 1997;94:12758-12760.

Schwer B, Linder P, Shuman S. (1998) Effects of deletion mutations in the yeast Ces1 protein on cell growth and morphology and on high copy suppresion of mutations in mRNA capping enzyme and translation initiation factor 4A. Nucleic Acids Res. 1998;26:803-809.

Ho CK, Sriskanda V, McCracken S, Bentley D, Schwer B, Shuman S. (1998) The guanylyltransferase domain of mammalian mRNA capping enzyme binds to the phosphorylated carboxyl-terminal domain of RNA polymerase II. J Biol Chem. 1998;273:9577-9585.

Schwer B, Mao X, Shuman S. (1998) Accelerated mRNA decay in conditional mutants of yeast mRNA capping enzyme. Nucleic Acids Res. 1998;26:2050-2057.

Gross CH, Shuman S. (1998) Characterization of a baculovirus-encoded RNA 5'-triphosphatase. J Virol. 1998;72:7057-7063.

Gross CH, Shuman S. (1998) RNA 5'-triphosphatase, nucleoside triphosphatase, and guanylyltransferase activities of baculovirus LEF-4 protein. J Virol. 1998;72:10020-10028.

Yu L, Martins A, Deng L, Shuman S. (1997) Structure-function analysis of the triphosphatase component of vaccinia virus mRNA capping enzyme. J Virol. 1997;71:9837-9843.

Ho CK, Pei Y, Shuman S. (1998) Yeast and viral RNA 5' triphosphatases comprise a new nucleoside triphosphatase family. J Biol Chem. 1998;273:34151-34156.

Wang SP, Shuman S. (1997) Structure-function analysis of the mRNA cap methyltransferase of Saccharomyces cerevisiae. J Biol Chem. 1997;272:14683-14689.

Ho CK, Lehman K, Shuman S. (1999) An essential surface motif (WAQKW) of yeast RNA triphosphatase mediates formation of the mRNA capping enzyme complex with RNA guanylyltransferase. Nucleic Acids Res. 1999;27:4671-4678.

Saha N, Schwer B, Shuman S. (1999) Characterization of human, Schizosaccharomyces pombe and Candida albicans mRNA cap methyltransferases and complete replacement of the yeast capping apparatus by mammalian enzymes. J Biol Chem. 1999;274:16553-16552.

Lehman K, Schwer B, Ho CK, Rouzankina I, Shuman S. (1999) A conserved domain of yeast RNA triphosphatase flanking the catalytic core regulates self-association and interaction with the guanylyltransferase component of the mRNA capping apparatus. J Biol Chem. 1999;274:22668-22678.

Pei Y, Ho CK, Schwer B, Shuman S. (1999) Mutational analyses of yeast RNA triphosphatases highlight a common mechanism of metal-dependent NTP hydrolysis and a means of targeting enzymes to pre-mRNAs in vivo by fusion to the guanylyltransferase component of the capping apparatus. J Biol Chem. 1999;274:28865-28874.

Lima CD, Wang LK, Shuman S. (1999) Structure and mechanism of yeast RNA triphosphatase: an essential component of the mRNA capping apparatus. Cell. 1999;99:533-543.

Pei Y, Lehman K, Tian L, Shuman S. (2000) Characterization of Candida albicans RNA triphosphatase and mutational analysis of its active site. Nucleic Acids Res. 2000;28:1885-18892.

Ho CK, Martins A, Shuman S. (2000) A yeast-based genetic system for functional analysis of viral mRNA capping enzymes. J Virol. 2000;74:5486-5494.

Ho CK, Schwer B, Shuman S. (1998) Genetic, physical, and functional interactions between the triphosphatase and guanylyltransferase components of the yeast mRNA capping apparatus. Mol Cell Biol. 1998;18:5189-5198.

Schwer B, Shuman S. (1996) Multicopy suppressors of temperature-sensitive mutations of yeast mRNA capping enzyme. Gene Expr. 1996;5:331-344.

Cong P, Shuman S. (1993) Covalent catalysis in nucleotidyl transfer: a KTDG motif essential for enzyme-GMP complex formation by mRNA capping enzyme is conserved at the active sites of RNA and DNA ligases. J Biol Chem. 1993;268:7256-7260.

Cong P, Shuman S. (1993) RNA binding properties of vaccinia virus capping enzyme. J Biol Chem. 1993;268:7256-7260.

Schwer B, Shuman S. (1994) Mutational analysis of yeast mRNA capping enzyme. Proc Natl Acad Sci USA. 1994;91:4328-4332.

Mao X, Shuman S. (1994) Intrinsic RNA (guanine-7) methyltransferase activity of the vaccinia virus capping enzyme D1 subunit is stimulated by the D12 subunit: Identification of amino acid residues in the D1 protein required for subunit association and methyl group transfer. J Biol Chem. 1994;269:24472-24479.

Shuman S, Liu Y, Schwer B. (1994) Covalent catalysis in nucleotidyl transfer reactions: essential motifs in S. cerevisiae RNA capping enzyme are conserved in Schizosaccharomyces pombe and viral capping enzymes and among polynucleotide ligases. Proc Natl Acad Sci USA. 1994;91:12046-12050.

Shuman S, Schwer B. (1995) RNA capping enzyme and DNA ligase - a superfamily of covalent nucleotidyl transferases. Mol Microbiol. 1995;17:405-410.

Mao X, Schwer B, Shuman S. (1995) Yeast mRNA cap methyltransferase is a 50-kDa protein encoded by an essential gene. Mol Cell Biol. 1995;15:4167-4174.

McCracken S, Fong N, Rosonina E, Yankulov K, Brothers G, Siderovski D, Hessel A, Foster S, Shuman S, Bentley DL. (1997) 5'-Capping enzymes are targeted to pre-mRNA by binding to the phosphorylated carboxy-terminal domain of RNA polymerase II. Genes Dev. 1997;11:3306-3318.

Mao X, Schwer B, Shuman S. (1996) Mutational analysis of the Saccharomyces cerevisiae ABD1 gene: cap methyltransferase activity is essential for cell growth. Mol Cell Biol. 1996;16:475-480.

Hagler J, Shuman S. (1992) A freeze-frame view of eukaryotic transcription during elongation and capping of nascent mRNA. Science. 1992;255:983-986.

Schwer B, Shuman S. (1996) Conditional inactivation of mRNA capping enzyme affects yeast pre-mRNA splicing in vivo. RNA. 1996;2:574-583.

Yu L, Shuman S. (1996) Mutational analysis of the triphosphatase domain of vaccinia virus mRNA capping enzyme. J Virol. 1996;70:6162-6168.

Ho CK, Van Etten JL, Shuman S. (1996) Expression and characterization of an RNA capping enzyme encoded by Chlorella virus PBCV-1. J Virol. 1996;70:6658-6664.

Mao X, Shuman S. (1996) Vaccinia virus mRNA (guanine-7-)methyltransferase: mutational effects on cap methylation and AdoHcy-dependent photo-cross-linking of the cap to the methyl acceptor site. Biochemistry. 1996;35:6900-6910.

Shuman S. (1997) A proposed mechanism of mRNA synthesis and capping by vesicular stomatitis virus. Virology. 1997;227:1-6.

Hakansson K, Doherty AJ, Shuman S, Wigley DB. (1997) X-ray crystallography reveals a large conformational change during guanyl transfer by mRNA capping enzymes. Cell. 1997;89:545-553.

Wang SP, Deng L, Ho CK, Shuman S. (1997) Phylogeny of mRNA capping enzymes. Proc Natl Acad Sci USA. 1997;94:9573-9578.

Cong P, Shuman S. (1995) Mutational analysis of mRNA capping enzyme identifies amino acids involved in GTP binding, enzyme-guanylate complex formation, and GMP transfer to RNA. Mol Cell Biol. 1995;15:6222-6231.

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