SK 851. Anti-HuR Monoclonal Antibodies

Summary of Invention

Cellular factors that bind to and regulate messenger RNA stability are important players in post-transcriptional control of gene expression. The HuR protein is one such factor; it binds to the 3'-untranslated region, specifically enhancing the stability or increasing the rate of turnover of mRNAs that encode oncogene products, cytokines, and growth factors.

Advantages

This invention contains the first available monoclonal antibody that recognizes the HuR protein and provides a critical reagent for investigation of HuR mechanism of action and function in regulation of RNA stability. The antibody detects human, mouse, rat, xenopus, and avian HuR. Assays, such as western blotting, immunocytochemistry, immunoprecipitation, and gel shift analysis, are made possible by these monoclonal antibodies.

Areas of Application

Research tool.

Stage of Development

Hybridoma cell line is available and may be obtained from Memorial Sloan-Kettering.

Lead Inventor

Dr. Henry M. Furneaux

References

• Manuscript detailing the use of this antibody is currently in preparation.
• Ma WJ, et al. J Biol Chem. 271(14):8144-51 (1996).
• Ma WJ, Furneaux HM. Hum Genet. 99(1):32-33 (1997).
• Levy NS, et al. J Biol Chem. 273(11):6417-23 (1998).

Contact Information:

Viviane Martin, PhD
Tel: 212-639-6181; 212-717-3934
E-mail: martinv@mskcc.org