Summary of Invention
We have determined and identified locus-specific sequences of introns 1, 2, and 3 from the majority of HLA-A, -B and -C allotypes and from alleles of HLA-E, -F, -G, -J, -K, and -L. These intron sequences are useful as primers for locus-specific amplification of the entirety of exons 2 and 3, i.e., the portion of these genes most suitable for use in typing of HLA-A, -B and -C. These primers are also suitable for use as sequencing primers to determine the HLA alleles in sequence-based HLA typing.
Advantages
Provides sequences of primers that are suitable for PCR-based sequence-specific oligonucleotide probe hybridization; sequence-specific primer-based amplification; and sequencing for HLA-A, - B and -C typing.
Areas of Application
Research tool.
Lead Inventor
Dr. Soo Y. Yang
Patent Information
US patent 6,030,775.
References
Contact Information
Yashodhara Dash, MBBS, PhD, MBA
Tel. 212-639-6181; Fax212-717-3439
E-mail: dashy@mskcc.org
