Summary of InventionWe have determined and identified locus-specific sequences of introns 1, 2, and 3 from the majority of HLA-A, -B and -C allotypes and from alleles of HLA-E, -F, -G, -J, -K, and -L. These intron sequences are useful as primers for locus-specific amplification of the entirety of exons 2 and 3, i.e., the portion of these genes most suitable for use in typing of HLA-A, -B and -C. These primers are also suitable for use as sequencing primers to determine the HLA alleles in sequence-based HLA typing. AdvantagesProvides sequences of primers that are suitable for PCR-based sequence-specific oligonucleotide probe hybridization; sequence-specific primer-based amplification; and sequencing for HLA-A, - B and -C typing. Areas of ApplicationResearch tool. Lead InventorDr. Soo Y. Yang Patent InformationUS patent 6,030,775. References
Contact InformationYashodhara Dash, MBBS, PhD, MBA Return to Research Tools Main Page
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