DNA Sequencing -- Core Facility

Agnes Viale (Core Facility Head)
E-mail(s)
genomics@mskcc.org

The Genomics core at Memorial Sloan-Kettering Cancer Center uses state-of-the-art technology and methodology to produce high quality DNA sequences. We support Sanger sequencing by capillary electrophoresis, pyrosequencing (Biotage), as well as next generation sequencing on 454-Titanium and SOLiD 4.0 sequencing systems. Our services includes consultation on experimental design, DNA extraction, library preparation, data export, primary and secondary data analysis (service provided through the bioinformatics core).

Sanger Sequencing

The Genomics core at Memorial Sloan-Kettering provides investigators access to automated DNA sequencing technology. The Core processes samples on 3730XL/3730 sequencers. Samples are typically processed in 24 hours, and yield 700 to 900 nucleotides of high-quality DNA sequence data. The cost for this service is $5.00 per sample. Fragment analysis is also available through the Genomics core.

454 and SOLiD sequencing

Since 2006, The Genomics Core also support two “next generation sequencing” platforms: the 454-Roche long reads sequencing system and the SOLiD (Applied Biosystems) short read sequencing system. Our services are comprehensive, from DNA shearing through data generation.

If you are interested in our services, please contact us at genomics@mskcc.org.