We are participating in the NIH Molecular Libraries Program (MLP) under our grant entitled “Diversity-Oriented Synthesis of Natural Product-based Libraries for the MLSMR” (P41 GM076267). Our novel natural product-based libraries are being submitted to the NIH Molecular Libraries Small Molecule Repository (MLSMR), where they are available to the scientific community for high-throughput screening in the NIH Molecular Libraries Probe Production Centers Network (MLPCN). Investigators interested in MLPCN screening under the R03 mechanism should see program announcement PAR-09-129. The results of these screens are available to the public via the PubChem database.
We using diversity-oriented synthesis to produce several different natural product-based libraries for the MLSMR. Some of these libraries are being synthesized in collaboration with Dr. Lisa Marcaurelle and other members of the Chemical Biology Platform at the Broad Institute.
A list of our compounds that are currently available for screening in the MLPCN (updated quarterly) can be accessed via the following LIVE SEARCH LINKS:
SK1 Spiroketal Library (Aliphatic)
(48 compounds submitted)
SK2 Spiroketal Library (Benzannulated)
(68 compounds submitted)
SK3 Spiroketal Library (Functionalized Benzannulated)
(30 compounds submitted)
PK1 Polyketide Fragment Library
(74 compounds submitted)
AT1 Alkaloid/Terpenoid Library (Multiscaffold)
(94 compounds submitted)
AT2 Alkaloid/Terpenoid Library (Pyrrolidinopyridines, 2-position full matrix)
(29 compounds submitted)
AT3 Alkaloid/Terpenoid Library (Pyrrolidinopyridines, 2-position sparse matrix)
(31 compounds submitted)
AT4 Alkaloid/Terpenoid Library (Pyrrolidinopyridines, 3-position sparse matrix)
(62 compounds submitted)
MC1 Macrocycle Library (Macrolides and Macrolactams)
(18 compounds submitted)
Screening results for these libraries are appearing regularly. To access screening data, click on one of the live search links above, then click on the Bioactivity Analysis button on the search results page
Full experimental details on the synthesis and purification of these compounds have been published previously in the following papers:
Spiroketal Diversity-Oriented Synthesis Libraries
These rigid, stereochemically diverse scaffolds from natural products present sidechains along well-defined three-dimensional vectors.
Wurst, J. M.; Liu, G.; Tan, D. S.* “Hydrogen-bonding catalysis and inhibition by simple solvents in the stereoselective kinetic epoxide-opening spirocyclization of glycal epoxides to form spiroketals.” J. Am. Chem. Soc. 2011, 133, 7916-7925.
[ Abstract | PDF | Supporting Info ]
Liu, G.; Wurst, J. M.; Tan, D. S.* “Stereoselective synthesis of benzannulated spiroketals: Influence of the aromatic ring on reactivity and conformation.” Org. Lett. 2009, 11, 3670-3673.
[ Abstract | PDF | Supporting Info | PMC ]
Moilanen, S. B.; Potuzak, J. S.; Tan, D. S.* “Stereocontrolled synthesis of spiroketals via Ti(Oi-Pr)4-mediated kinetic spirocyclization of glycal epoxides with retention of configuration.” J. Am. Chem. Soc. 2006, 128, 1792-1793.
[ Abstract | PDF | Supporting Info | PMC ]
Highlighted in Nature
Potuzak, J. S.; Moilanen, S. B.; Tan, D. S.* “Stereocontrolled synthesis of spiroketals via a remarkable methanol-induced kinetic spirocyclization reaction.” J. Am. Chem. Soc. 2005, 127, 13796-13797.
[ Abstract | PDF | Supporting Info ]
Moilanen, S. B.; Tan, D. S.* “Enantioselective synthesis of erythro-4-deoxyglycals as scaffolds for target- and diversity-oriented synthesis: New insights into glycal reactivity.” Org. Biomol. Chem. 2005, 3, 798-803.
[ Abstract | PDF | Supporting Info ]
Potuzak, J. S.; Tan, D. S.* “Synthesis of C1-alkyl and C1-acylglycals from glycals using a B-alkyl Suzuki–Miyaura cross coupling approach.” Tetrahedron Lett. 2004, 45, 1797-1801.
[ Abstract | PDF | Supporting Info ]
Polyketide Diversity-Oriented Synthesis Libraries
These structural motifs from natural products exhibit skeletal and conformational diversity.
Shang, S.; Iwadare, H.; Macks, D. E.; Ambrosini, L. M.; Tan, D. S.* “A unified synthetic approach to polyketides having both skeletal and stereochemical diversity.” Org. Lett. 2007, 9, 1895-1898.
[ Abstract | PDF | Supporting Info | PMC ]
Alkaloid/Terpenoid Diversity-Oriented Synthesis Libraries
This multiscaffold library is comprised of polycyclic structures related to alkaloid and terpenoid natural products.
Moura-Letts, G.; DiBlasi, C. M.; Bauer, R. A.; Tan, D. S.* “Solid-phase synthesis and chemical space analysis of a 190-membered alkaloid/terpenoid-like library.” Proc. Natl. Acad. Sci. USA 2011, 108, 6745-6750.
[ Abstract | PDF | Supporting Info ]
Bauer, R. A.; DiBlasi, C. M.; Tan, D. S.* “The tert-butylsulfinamide lynchpin in transition-metal-mediated multiscaffold library synthesis.” Org. Lett. 2010, 12, 2084-2087.
[ Abstract | PDF | Supporting Info | PMC ]
TBDAS Linker for Solid Phase Diversity-Oriented Synthesis
This robust linker is stable to a wide range of organic reaction conditions and is cleaved chemoselectively with fluoride reagents. Click here for more details.
DiBlasi, C. M.; Macks, D. E.; Tan, D. S.* “An acid-stable tert-butyldiarylsilyl (TBDAS) linker for solid-phase organic synthesis.” Org. Lett. 2005, 7, 1777-1780.
[ Abstract | PDF | Supporting Info ]
Highlighted in Letters in Organic Chemistry [PDF]
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