The focus of my work is to use bacterial artificial chromosome (BAC) transgenic embryonic stem cells (ESCs) that express GFP under promoters that are specific to midbrain dopamine (mDA) neurons in order to purify, characterize, and graft these cells in a mouse model of Parkinson's disease. I also work with the Rutishauser lab to overexpress polysialic acid neural cell adhesion molecule (PSA-NCAM) in grafted ESC-derived mDA neurons to increase their integration into the host CNS.
Publications by Yosif Martin Ganat
Identification of embryonic stem cell–derived midbrain dopaminergic neurons for engraftment. Ganat YM, Kriks S, Calder E, Tu Y, Jia F, Harrison N, Tomishima MJ, Rutishauser U and Studer L. Journal of Clinical Investigation, 2012 Jul 2. pii: 58767.
Dopamine neurons derived from human ES cells efficiently engraft in animal models of Parkinson's disease. Kriks S, Shim JW, Piao J, Ganat YM, Wakeman DR, Xie Z, Carrillo-Reid L, Auyeung G, Antonacci C, Buch A, Yang L, Beal MF, Surmeier DJ, Kordower JH, Tabar V, Studer L. Nature. 2011 Nov 6;480(7378):547-51. doi: 10.1038/nature10648.
miR-371-3 expression predicts neural differentiation propensity in human pluripotent stem cells. Kim H, Lee G, Ganat Y, Papapetrou EP, Lipchina I, Socci ND, Sadelain M, Studer L. Cell Stem Cell. 2011 Jun 3;8(6):695-706.
Astroglial cells in the external granular layer are precursors of cerebellar granule neurons in neonates. Silbereis J, Heintz T, Taylor MM, Ganat Y, Ment LR, Bordey A, Vaccarino F. Mol Cell Neurosci. 2010 Aug;44(4):362-73. Epub 2010 May 12
Modelling pathogenesis and treatment of familial dysautonomia using patient-specific iPSCs. Lee G, Papapetrou EP, Kim H, Chambers SM, Tomishima MJ, Fasano CA, Ganat YM, Menon J, Shimizu F, Viale A, Tabar V, Sadelain M, Studer L. Nature. 2009 Sep 17;461(7262):402-6. Epub 2009 Aug 19.
Precursors with glial fibrillary acidic protein promoter activity transiently generate GABA interneurons in the postnatal cerebellum. Silbereis J, Cheng E, Ganat YM, Ment LR, Vaccarino FM. Stem Cells. 2009 May;27(5):1152-63.
Fgfr1 is required for cortical regeneration and repair after perinatal hypoxia. Fagel DM, Ganat Y, Cheng E, Silbereis J, Ohkubo Y, Ment LR, Vaccarino FM. J Neurosci. 2009 Jan 28;29(4):1202-11.
Astroglial cells in development, regeneration, and repair. Vaccarino FM, Fagel DM, Ganat Y, Maragnoli ME, Ment LR, Ohkubo Y, Schwartz ML, Silbereis J, Smith KM. Neuroscientist. 2007 Apr;13(2):173-85. Review.
Early postnatal astroglial cells generate multilineage precursors and neural stem cells in vivo. Ganat YM, Silbereis J, Cave C, Ngu H, Anderson JM, Ohkubo Y, Ment LR, Vaccarino FM. J Neurosci. 2006 Aug 16;26(33):8609-21.
Cortical neurogenesis enhanced by chronic perinatal hypoxia. Fagel DM, Ganat Y, Silbereis J, Ebbitt T, Stewart W, Zhang H, Ment LR, Vaccarino FM. Exp Neurol. 2006 May;199(1):77-91.
Chronic hypoxia up-regulates fibroblast growth factor ligands in the perinatal brain and induces fibroblast growth factor-responsive radial glial cells in the sub-ependymal zone. Ganat Y. Soni S. Chacon M. Schwartz ML. Vaccarino FM. Neuroscience. 112(4):977-91, 2002.
Stem cells in neurodevelopment and plasticity. Vaccarino FM. Ganat Y. Zhang Y. Zheng W. Neuropsychopharmacology. 25(6):805-15, 2001 Dec.
