Immune Reconstitution After Allogeneic Hematopoietic Stem Cell Transplantation and Adoptive Immunotherapy

Prolonged immunodeficiency after allogeneic bone marrow transplantation results in significant morbidity and mortality from infection. A number of strategies to enhance immune reconstitution post-transplant are being explored.

In Vivo Imaging Techniques

In vivo imaging techniques, such as bioluminescence imaging (BLI) and positron emission tomography (PET) allows the study of growth and migration of selected cell populations in a single animal over time.

GVHD and the Microbiota

In collaboration with Eric Pamer and colleagues, we are using 16s rRNA gene sequencing techniques to characterize how the microbiota can interact with GVHD.

Adoptive Immunotherapy with Lymphoid Precursor Cells

Culture systems utilizing Notch1 signaling can be used for the in vitro development of T lineage cells at various differentiation stages.

Homing of T cells in Graft-versus-host Disease

Donor T cells play a pivotal role in graft-versus-tumor (GVT) activity and graft-versus-host disease (GVHD) after HSCT. Although alloantigens are omnipresent in the recipient, GVHD is limited to a few organs (liver, intestines, and skin), and this selectivity is poorly understood.

Absence of P-selectin in Recipients of Allogeneic Bone Marrow Transplantation Ameliorates Experimental Graft-versus-Host-Disease

Graft-versus-host-disease (GVHD) is a systemic inflammatory disease that specifically affects the gastrointestinal tract, liver and skin.

CEACAM1 Negatively Regulates Acute Graft-Versus-Host-Disease After Allogeneic Bone Marrow Transplantation

Carcinoembryonic antigen related cell adhesion molecule 1 (CEACAM1) negatively regulates T cell function.

STAT-3 and ERK 1/2 phosphorylation are critical for T cell alloactivation and Graft-Versus-Host-Disease

Graft-versus-host-disease (GVHD) is a serious complication of allogeneic bone marrow transplantation, and donor T cells are indispensable for GVHD. Current therapies have limited efficacy, selectivity, and high toxicities.