Molecular Cytology -- Core Facility

Katia Manova (Core Facility Head)
Office phone:
646-888-2173
Office fax:
646-422-0640

Mission

  • Identify and master technologies for detection and analysis of molecules in their natural environment (in cells, tissues, organs, embryos and tumors).
  • Stay abreast of new technological developments and continue to expand and enhance services provided by the Core.
  • Provide consultations, training and technical assistance in the execution of the technologies.
  • Process samples for in situ molecular detection.

Automated Experiments

Please visit our internal website and image gallery. Molecular Cytology Core Facility uses state-of-the-art machines with a capacity of 160 slides a day in order to service users' experiments. The experiments include:

in situ protein detection of by IHC/IF (single, double, triple and more) with markers for:

RNA detection by in situ hybridization (messenger and micro RNA)

Methods used are:

  • Immunohistochemistry (single/double)
  • Immunofluorescence (single/double/triple)
  • Non-radioactive RNA in situ hybridization
  • Double non-radioactive RNA in situ hybridization and immunohistochemistry

Our purpose is to automate time- consuming and cumbersome manual experiments, achieve results with high quality and reproducibility, and continue to expand the list of in situ detection protocols in synchrony with introduction of new molecular markers and with user needs.

Please visit click here to view the list of validated antibodies.

Manual Experiments

Please visit our internal website and image gallery. Our staff will train and guide you through all the steps of tissue processing, sample preparation and manual in situ detection of molecules of your interest.

  • Tissue fixation
  • Embedding tissues in paraffin, OCT, agarose and gelatin
  • Preparing paraffin, cryo and vibratome sections
  • Histological staining (e.g. H&E, Cresyl Violet, PAS)
  • Manual in situ molecular detection (e.g. TUNEL, β-gal, IHC/IF, mRNA, microRNA)
  • Sample preparation for imaging

Digital Microscopy and Image Analysis

Please visit our internal website and imaging and image analysis information. We assist our users to design, prepare samples, execute and analyze imaging experiments, prepare samples, execute and analyze imaging experiments.

  • Laser-scanning confocal microscopes for optical sectioning
  • Wide-field microscopes for epi-fluorescent and brightfield imaging
  • Stereoscopic microscopes
  • Laser capture microdissection
  • Complete digitization of stained slides (bright field and fluorescence)
  • Spectral separation
  • Live imaging
  • Time-lapse imaging
  • High-level imaging techniques (FRAP, FRET, FLIM)
  • Image processing and analysis
Publications