Antigen: Mouse CD45.2 (Ly5.2)
Clone Name: 104-2
Isotype: Mouse IgG2a (Kappa Light Chain)
Application(s)*: Flow Cytometry, Immunofluorescence Microscopy, Immunoprecipitation
* As reported in the literature and other commercial supplier websites
Clone 104-2 reacts with CD45 (Leukocyte Common Antigen) on leukocytes of mouse strains that express the CD45.2 alloantigen, including A, AKR, BALB⁄c, CBA⁄Ca, CBA⁄J, C3H⁄He, C57BL, C57BR, C57L, C58, DBA⁄1, DBA⁄2, NZB, SWR, and 129. It has been reported not to react with leukocytes from mouse strains expressing the CD45.1 alloantigen.
This antibody was derived in 1981 by injection of thymocytes and splenocytes from B10.S mice into SJL mice. Splenocytes from these SJL mice were fused with NS-1 cells to generate hybridomas.
- Edward Boyse, MD, formerly of Memorial Sloan Kettering
- Fung-Win Shen, PhD
- Shen FW (1981) Monoclonal antibodies to mouse lymphocyte differentiation alloantigens. Monoclonal Antibodies and T-Cell Hybridomas: Perspectives and Technical Advances. Hämmerling GJ, Hämmerling U and Kearney JF, editors. Elsevier/North-Holland Biomedical Press, Amsterdam. 25-31 (ISBN: 9780444803511)
- Yakura H et al. (1983) On the function of Ly-5 in the regulation of antigen-driven B cell differentiation. Comparison and contrast with Lyb-2. Journal of Experimental Medicine 157: 1077-88 (PubMed ID: 6220106)
This hybridoma may be licensed nonexclusively for commercial purposes. Please note that Anti-CD45.1 Mouse Monoclonal Antibody (Clone A-20) is also available for licensing from MSK.
Tingting Zhang-Kharas, PhD
Purified antibody may be available for sale through the Memorial Sloan Kettering Monoclonal Antibody Core Facility. Please email firstname.lastname@example.org for further information.