Antigen: Mouse NK1.1 (CD161, NKR-P1C, Ly-55)
Clone Name: PK136
Isotype: Mouse IgG2a (Kappa Light Chain)
Application(s)*: Flow Cytometry, Immunoprecipitation, Immunohistochemistry, Immunofluorescence
* As reported in the literature and other commercial supplier websites
Clone PK136 recognizes mouse NK1.1, a cell surface antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including CE, C57BL/6, FVB/N, and NZB. NK1.1 is not expressed by NK cells from the following mouse strains: 129, A, AKR, BALB/c, C3H, CBA, and SJL.
This antibody was derived in 1984 by injection of splenocytes (enriched for NK-1-positive cells) and bone marrow cells from CE mice into (C3H x BALB/c) F1 mice. Splenocytes from these mice were then fused with Sp2/0-Ag14 cells to generate hybridomas.
- Gloria C. Koo, PhD, formerly at Memorial Sloan Kettering
- JoAnne R. Peppard, formerly at Memorial Sloan Kettering
Koo GC and Peppard JR (1984) Establishment of monoclonal anti-Nk-1.1 antibody. Hybridoma
3: 301-303 (PubMed ID: 6500587
Koo GC et al. (1986) The NK-1.1(-) mouse: a model to study differentiation of murine NK cells. Journal of Immunology.
137: 3742-3747 (PubMed ID: 3782794
Reichlin A and Yokoyama WM (1998) Natural killer cell proliferation induced by anti-NK1.1 and IL-2. Immunology and Cell Biology
76: 143-152 (PubMed ID: 9619484
Kung SK et al. (1999) The NKR-P1B gene product is an inhibitory receptor on SJL/J NK cells. Journal of Immunology
162: 5876-5887 (PubMed ID: 10229823
This hybridoma may be licensed nonexclusively for commercial purposes.
Tingting Zhang-Kharas, PhD
Purified antibody may be available for sale through the Memorial Sloan Kettering Monoclonal Antibody Core Facility. Please email firstname.lastname@example.org for further information.