Tangible Material: HT-144: Human Melanoma Cell Line

SK1980-544

 

Description

HT-144 is a malignant human melanoma cell line that displays aneuploid fibroblastic morphology and grows in adherent tissue culture. This cell line has been reported to be nonpermissive for human cytomegalovirus (HCMV). HT-144 cells form xenograft tumors when injected into immunocompromised mice. These cells contain a mutation in the ATM gene, resulting in the expression of a truncated protein, which causes increased sensitivity to UVB and ionizing radiation compared to other melanoma cell lines. The HT-144 cells also express mutant B-Raf (V600E).

Source

This cell line was established in 1966 from a metastatic site (subcutaneous tissue) in a 29-year-old Caucasian male with malignant melanoma.

Inventors

  • Jorgen Fogh, PhD, formerly at Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
  • Germaine Trempe, formerly at Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center

Key References

·       Fogh J et al. (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. Journal of the National Cancer Institute 59: 221-226 (PubMed ID: 327080)
·       Smith JD (1986) Human cytomegalovirus: demonstration of permissive epithelial cells and nonpermissive fibroblastic cells in a survey of human cell lines. Journal of Virology 60: 583-588 (PubMed ID: 3021992)
·       Ramsay J et al. (1998) Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia. British Journal of Cancer 77: 11-14 (PubMed ID: 9459139)
·       Chen B et al. (2012) BRAFV600E negatively regulates the AKT pathway in melanoma cell lines. PLoS One 7: e42598 (PubMed ID: 22880048)

Licensing Information

This cell line may be licensed nonexclusively for research or commercial purposes.

Contact Information

Tingting Zhang-Kharas, PhD
Licensing Assistant
Tel: 646-888-1083; Fax: 646-888-1120

E-mail: zhangkht@mskcc.org

Stage of Development
Ready to use
Technology Types
Indications