HT-144 is a malignant human melanoma cell line that displays aneuploid fibroblastic morphology and grows in adherent tissue culture. This cell line has been reported to be nonpermissive for human cytomegalovirus (HCMV). HT-144 cells form xenograft tumors when injected into immunocompromised mice. These cells contain a mutation in the ATM gene, resulting in the expression of a truncated protein, which causes increased sensitivity to UVB and ionizing radiation compared to other melanoma cell lines. The HT-144 cells also express mutant B-Raf (V600E).
This cell line was established in 1966 from a metastatic site (subcutaneous tissue) in a 29-year-old Caucasian male with malignant melanoma.
- Jorgen Fogh, PhD, formerly at Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
- Germaine Trempe, formerly at Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
· Fogh J et al. (1977) One hundred and twenty-seven cultured human tumor
cell lines producing tumors in nude mice. Journal of the National Cancer Institute
59: 221-226 (PubMed ID: 327080
· Smith JD (1986) Human cytomegalovirus: demonstration of permissive epithelial
cells and nonpermissive fibroblastic cells in a survey of human cell lines. Journal of Virology
60: 583-588 (PubMed ID: 3021992
· Ramsay J et al. (1998) Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia
. British Journal of Cancer
77: 11-14 (PubMed ID: 9459139
· Chen B et al. (2012) BRAFV600E negatively regulates the AKT pathway in melanoma cell lines. PLoS One
7: e42598 (PubMed ID: 22880048
This cell line may be licensed nonexclusively for research or commercial purposes.
Tingting Zhang-Kharas, PhD