Methods and Reagents for Typing HLA Class I Genes

Summary of Invention

We have determined and identified locus-specific sequences of introns 1, 2, and 3 from the majority of HLA-A, -B and -C allotypes and from alleles of HLA-E, -F, -G, -J, -K, and -L. These intron sequences are useful as primers for locus-specific amplification of the entirety of exons 2 and 3, i.e., the portion of these genes most suitable for use in typing of HLA-A, -B and -C. These primers are also suitable for use as sequencing primers to determine the HLA alleles in sequence-based HLA typing.

Advantages

Provides sequences of primers that are suitable for PCR-based sequence-specific oligonucleotide probe hybridization; sequence-specific primer-based amplification; and sequencing for HLA-A, - B and -C typing.

Areas of Application

Research tool

Lead Inventor

Soo Y. Yang, PhD

Patent Information

U.S. patent issued: 6,030,775

References

• Cereb N, et al. (1995) Tissue Antigens. 45:1-11
• Cereb N, et al. (1996) Tissue Antigens. 47:498-511
• Cereb N, Yang SY. (1997) Tissue Antigens. 50:74-76

Contact Information