During translation messenger RNA produced by transcription is decoded by ribosomes to produce specific polypeptides. Recently, a second generation sequencing methodology was developed called ribosomal profiling that measures the positions and counts of ribosomes. When combined with corresponding mRNA sequencing data, ribosomal data can give insights into translational efficiency. I will discuss issues that arise when analyzing such data, including normalization and testing hypotheses.
This is joint work with Barry Taylor and Richard Olshen.