The investigators have developed a novel method of directly measuring human T Cell Receptor (TCR) diversity with high resolution and accuracy.
A diverse TCR repertoire is capable of recognizing a broad range of antigens present on pathogens and is a necessary component of an effective immune system. Knowing a patient’s exact TCR repertoire would allow researchers and clinicians to understand immune physiology, and monitor disease progression and drug response. Such analyses can help identify patients with a higher likelihood to respond to immunotherapy. Additionally, such knowledge could lead to the development and adoption of strategies that improve T cell reconstitution, greatly reducing transplant-associated morbidity and mortality and the health risks associated with other auto-immune diseases.
- This technology combines two techniques, 5′ RACE PCR and deep sequencing, in order to determine TCR repertoire complexity with great precision. This novel approach allows for a more accurate measure of T cell receptors than currently used methods, which only provide a rough estimate of repertoire complexity.
- This method can also assess the entire TCR-β repertoire using a single oligonucleotide pair, thereby eliminating amplification bias and the resulting inaccuracy often associated with PCR.
With a potential market as a research tool or diagnostic, this method can be used to characterize the immunity of a patient provided by T cells in clinical settings of allo-HSCT, immune deficiency, autoimmunity, and tumor immunity.
Monitoring immune state
PCT application published, PCT/US2013/049404; national applications pending in U.S., Canada, and Europe