Analysis of T Cell Receptor Repertoire

SK2012-042

Summary of Invention

The investigators have developed a novel method of directly measuring human T Cell Receptor (TCR) diversity with high resolution and accuracy.

A diverse TCR repertoire is capable of recognizing a broad range of antigens present on pathogens and is a necessary component of an effective immune system. Knowing a patient’s exact TCR repertoire would allow researchers and clinicians to understand immune physiology, and monitor disease progression and drug response. Such analyses can help identify patients with a higher likelihood to respond to immunotherapy. Additionally, such knowledge could lead to the development and adoption of strategies that improve T cell reconstitution, greatly reducing transplant-associated morbidity and mortality and the health risks associated with other auto-immune diseases.

Advantages

  • This technology combines two techniques, 5′ RACE PCR and deep sequencing, in order to determine TCR repertoire complexity with great precision. This novel approach allows for a more accurate measure of T cell receptors than currently used methods, which only provide a rough estimate of repertoire complexity.
  • This method can also assess the entire TCR-β repertoire using a single oligonucleotide pair, thereby eliminating amplification bias and the resulting inaccuracy often associated with PCR.

Market Opportunities

With a potential market as a research tool or diagnostic, this method can be used to characterize the immunity of a patient provided by T cells in clinical settings of allo-HSCT, immune deficiency, autoimmunity, and tumor immunity.

Areas of Application

Monitoring immune state

Patent Information

PCT application published, PCT/US2013/049404; national applications pending in U.S., Canada, and Europe

Lead Investigator

Dr. Eric Pamer, MD, Laboratory Head, Immunology Program, Sloan Kettering Institute, Memorial Sloan Kettering

Contact Information

Kannan Krishnamurthy, PhD
Licensing Manager
Tel: 646-888-1075

Stage of Development

Ready to use