MSK-PCa1 is a human prostate cancer organoid cell line. In culture, the tumor cells formed organoids that displayed an intra-ductal pattern reminiscent of the primary cancer. IHC showed that the organoids were positive for pan-cytokeratin and negative AR, recapitulating the biopsy specimen. It could be grafted in SCID mice and tumors formed that recapitulated both the histological and immunohistological pattern of the patient sample. The established organoid line exhibits a double time of 3 days.
MSK-PCa1 is derived from a patient who underwent radical prostatectomy for a Gleason 4+5 adenocarcinoma with prominent intraductal features. He developed a metastatic lesion in the L2 vertebral body two months after surgery and was treated with androgen deprivation therapy (ADT) and radiotherapy. Six months later, the lesion progressed despite castrate level of testosterone and a nondetectable PSA. A biopsy was taken during a kyphoplasty procedure and was cultured.
- Yu Chen, MD, PhD, Human Oncology and Pathogenesis Program, Department of Medicine, Memorial Sloan Kettering Cancer Center
- Dong Gao, PhD, formerly at Human Oncology and Pathogenesis Program, Department of Medicine, Memorial Sloan Kettering Cancer Center
Gao D, et al. Organoid Cultures Derived from Patients with Advanced Prostate Cancer. Cell, 2014;159(1):176-87.
This organoid cell line may be licensed nonexclusively for research or commercial purposes.