Cultured organoids showed typical small cell morphology with high nucleus to cytoplasm ratio that stained positive for synaptophysin. Organoids with weaker synaptophysin staining showed perinuclear pancytokeratin staining typical of small cell prostate cancer, while those with stronger synaptophysin staining were pan-cytokeratin negative. When grafted into immuno-compromised mice, the tumors exhibited typical small cell histology with high nuclear to cytoplasmic ratio and frequent mitotic figures. The established organoid line exhibits a double time of 2 weeks
MSK-PCa4 is developed from a patient with Gleason 4+3 adenocarcinoma seven years after initial diagnosis and treatment with combined ADT and radiotherapy. The restarted ADT with progression of disease five years after diagnosis, subsequently progressed despite PSA of < 1 ng/dl. A malignant pleural effusion showed prostate cancer with neuroendocrine differentiation that was positive for synaptophysin.
- Yu Chen, MD, PhD, Human Oncology and Pathogenesis Program, Department of Medicine, Memorial Sloan Kettering Cancer Center
- Dong Gao, PhD, formerly at Human Oncology and Pathogenesis Program, Department of Medicine, Memorial Sloan Kettering Cancer Center
Gao D, et al. Organoid Cultures Derived from Patients with Advanced Prostate Cancer. Cell, 2014;159(1):176-87.
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