More About The Danwei Huangfu Lab Minus iconIcon indicating subtraction, or that the element can be closed. Plus IconIcon indicating addition, or that the element can be opened. Arrow (down) icon.An arrow icon, usually indicating that the containing element can be opened and closed.

Reagents and protocols

Plasmids

Request plasmids through Addgene.

Cell lines

The following hPSC lines generated by the Huangfu lab are distributed by the Monoclonal Antibody Facility at MSKCC (skiabcf@mskcc.org):

◦     HUES8 iCas9 (González et al. 2014)
◦     HUES9 iCas9 (González et al. 2014)
◦     MEL1 iCas9 (González et al. 2014)
◦     H1 iCas9 (Shi et al. 2016)
◦     H9 iCas9 (Verma et al., unpublished)
◦     CV iPSC iCas9 (Shi et al., unpublished)
◦     BJ iPSC iCas9 (González et al. 2014)
 
◦     PDX1-GFP knockin reporter line (Zhu et al. 2015)
◦     OCT4-GFP knockin reporter line (Zhu et al. 2015)
◦     OCT4-mOrange knockin reporter line (Zhu et al. 2015)
 

Protocols

CRISPR/Cas-Mediated Knockin in Human Pluripotent Stem Cells. Verma N, Zhu Z, Huangfu D. Methods Mol Biol. 2017;1513:119-140. PMID:27807834

CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium. Soh CL, Huangfu D. Methods Mol Biol. 2017;1498:57-78. PMID:27709569

The iCRISPR platform for rapid genome editing in human pluripotent stem cells. Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215-50. doi: 10.1016/B978-0-12-801185-0.00011-8. PMID:25398343