RNA Binding Proteins as Targets of Drug Therapy

In contrast, we have also looked at RNA binding proteins and their functions in controlling protein expression in drug resistant lines. We have isolated novel proteins in epithelial tumor cells which appear to mask mRNA from genes with basal transcription, preventing protein expression. We are currently utilizing pharmacophores that interfere with this process and release mRNA for protein expression. The regulation of this process is also under investigation.

Aliza L, Leiser L, Guangli Y, Huasong T, Springett G, Dabrowska M, Yan XJ, Rosales N, Bornmann W, Spriggs DR. Novel Tetrafluorodinated Phthalimide Analogues with ’are’ Dependent TNF-ALPHA Regulatory Activity. [In Press]

Wang E, Ma WJ, Aghajanian C, Spriggs DR. Posttranscriptional regulation of protein expression in human epithelial carcinoma cells by adenine-uridine-rich elements in the 3’-untranslated region of tumor necrosis factor-alpha messenger RNA. Cancer Res. 1997 Dec 1;57(23):5426-33.