Johannes L Zakrzewski

Assistant Attending Pediatrician and Assistant Member

Johannes L Zakrzewski

Lab Phone


I am working on strategies to enhance immune reconstitution and graft-versus-tumor
activity after hematopoietic stem cell transplantation (HSCT). My main focus is on T and
NK cell reconstitution, developing strategies based on adoptive transfer of in vitro
generated lymphoid precursor cells into syngeneic and allogeneic HSCT recipients.


K08 CA160659-05
Strategies to enhance thymus-independent T cell development in cancer patients

6465-15, Leukemia and Lymphoma Society Translational Research Program
Strategies to overcome transplantation barriers and treat hematologic malignancies enabled by small molecule-mediated inhibition of the transcription factor c-Rel


University of Erlangen-Nurnberg, Germany


Zakrzewski JL et al. Adoptive transfer of T-cell precursors enhances Tcell reconstitution after allogeneic hematopoietic stem cell transplantation. Nat Med 2006; 12: 1039-1047.

Zakrzewski JL et al. Tumor immunotherapy across MHC barriers using allogeneic T cell precursors. Nature Biotechnol 2008; 26: 453-461.

Shono Y, Tuckett AZ, Ouk S, Liou HS, Altan-Bonnet G, Tsai JJ, Oyler JE, Smith OM, West ML, Singer NV, Doubrovina E, Pankov D, Unhad CV, Murphy GF, Lezcano C, Liu C, O’Reilly RJ, van den Brink MRM, Zakrzewski JL. A small molecule c-Rel inhibitor reduces alloactivation of T cells without compromising anti-tumor activity. Cancer Discovery 2014; 4: 578-591.

Tuckett AT, Thornton RH, Shono Y, Smith OM, Levy E, Kreines F, van den Brink RM, Zakrzewski JL. Image- guided intrathymic injection of multipotent stem cells supports life-long T cell immunity and facilitates targeted immunotherapy. Blood 2014; 123: 2797-2805.

Zakrzewski JL, van den Brink MRM, Hubbell JA. Overcoming immunological barriers in regenerative medicine. Nat Biotechnol 2014; 32:786-794.

My Projects