Plasmids
Request plasmids through Addgene.
Cell lines
The following hPSC lines generated by the Huangfu lab are distributed by the Monoclonal Antibody Facility at MSKCC ([email protected]):
◦ HUES8 iCas9 (González et al. 2014)
◦ HUES9 iCas9 (González et al. 2014)
◦ MEL1 iCas9 (González et al. 2014)
◦ H1 iCas9 (Shi et al. 2016)
◦ H9 iCas9 (Verma et al., unpublished)
◦ CV iPSC iCas9 (Shi et al., unpublished)
◦ CV iPSC iCas9 (Shi et al., unpublished)
◦ BJ iPSC iCas9 (González et al. 2014)
◦ PDX1-GFP knockin reporter line (Zhu et al. 2015)
◦ OCT4-GFP knockin reporter line (Zhu et al. 2015)
◦ OCT4-mOrange knockin reporter line (Zhu et al. 2015)
Protocols
CRISPR/Cas-Mediated Knockin in Human Pluripotent Stem Cells. Verma N, Zhu Z, Huangfu D. Methods Mol Biol. 2017;1513:119-140. PMID:27807834
CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium. Soh CL, Huangfu D. Methods Mol Biol. 2017;1498:57-78. PMID:27709569
The iCRISPR platform for rapid genome editing in human pluripotent stem cells. Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215-50. doi: 10.1016/B978-0-12-801185-0.00011-8. PMID:25398343