Reagents and Protocols

Plasmids

Request plasmids through Addgene.

Cell lines

The following hPSC lines generated by the Huangfu lab are distributed by the Monoclonal Antibody Facility at MSKCC (skiabcf@mskcc.org):

◦     HUES8 iCas9 (González et al. 2014)
◦     HUES9 iCas9 (González et al. 2014)
◦     MEL1 iCas9 (González et al. 2014)
◦     H1 iCas9 (Shi et al. 2016)
◦     H9 iCas9 (Verma et al., unpublished)
◦     CV iPSC iCas9 (Shi et al., unpublished)
◦     BJ iPSC iCas9 (González et al. 2014)
 
◦     PDX1-GFP knockin reporter line (Zhu et al. 2015)
◦     OCT4-GFP knockin reporter line (Zhu et al. 2015)
◦     OCT4-mOrange knockin reporter line (Zhu et al. 2015)
 

Protocols

CRISPR/Cas-Mediated Knockin in Human Pluripotent Stem Cells. Verma N, Zhu Z, Huangfu D. Methods Mol Biol. 2017;1513:119-140. PMID:27807834

CRISPR/Cas9-Mediated Mutagenesis of Human Pluripotent Stem Cells in Defined Xeno-Free E8 Medium. Soh CL, Huangfu D. Methods Mol Biol. 2017;1498:57-78. PMID:27709569

The iCRISPR platform for rapid genome editing in human pluripotent stem cells. Zhu Z, González F, Huangfu D. Methods Enzymol. 2014;546:215-50. doi: 10.1016/B978-0-12-801185-0.00011-8. PMID:25398343