Purpose

• Identify and master technologies for detection and analysis of molecules in their natural environment (in cells, tissues, organs, embryos and tumors).
• Stay abreast of new technological developments and continue to expand and enhance the research capabilities.
• Provide consultations, training and technical assistance in the execution of the technologies

Molecular Cytology View photos of the Molecular Cytology Core Facility start

Training and Technical Assistance In

1. Tissue Processing and Sample Preparation
   • Tissue fixation and embedding in paraffin, OCT and agarose
   • Preparing paraffin, cryosections and vibratome sections

2. In Situ Molecular Detection (Automated And Manual) (image gallery)

   • in situ mRNA Detection
   • Automated non-radioactive RNA in situ hybridization on paraffin, cryosections and embryos in whole mount (mouse, Drosophila, chick)
   • Manual radioactive RNA in situ hybridization on paraffin and cryosections
   • in situ Detection of Protein Expression, Cell Proliferation, Cell Death, Angiogenesis, Hypoxia
   • Automated immunohistochemistry and immunofluorescence (single and double)
   • Automated detection of BrdU, PCNA, Ki-67, P- Histone H3
   • Automated Tunel, cleaved caspase- 3, cleaved PARP
   • Automated detection of Pimonidazole adducts, HIF1 alpha
     
     (list of primary antibodies)

3. Digital Microscopy and Image Analysis (details)
   • Confocal Imaging: One-Photon, Two-Photon, Nipkow disc confocal microscope

   1. Leica TCS SP2 AOBS 1- and 2-photon laser scanning confocal (DMRXA2 upright stand)

   2. Leica TCS SP2 AOBS 1- and 2-photon laser scanning confocal (DM IRE2 inverted stand)

   3. Zeiss LSM510 laser scanning confocal (Axiovert 200M inverted stand)

   4. PerkinElmer UltraVIEW ERS Rapid Confocal Imager (Axiovert 200M inverted stand)

   • Wide-Field Imaging: Epifluorescence, Nomarski, Phase Contrast, Dark Field, Polarized Light, Structured Illumination (Apotome)

   1. Two Zeiss Axioplan 2 Imaging microscopes with ApoTome™ (upright stand)

   2. . Zeiss Axioplan 2 (upright stand)

   3. Zeiss Axiovert 200M 'Metamorph' (inverted stand)

   • Stereoscopic Imaging

   1. Leica MZ FLIII stereoscope

   • Live-Cell Imaging

   • Laser Microdissection

   • Arcturus Laser Capture Microdissection System (LCM)

   Methods

   • Quantitative Imaging and Image Analysis: 3D Reconstructions, Cell Population Counting, Colocalization, FRAP, FRET, FLIM, in situ Measurements of Length, Volume, Time, Localization at 200nm Resolution, Ratio Imaging, Relative Expression Rates, Spectral Imaging and Spectral Deconvolution