Research Articles Bio/Publications at ORCID: 0000-0002-9163-6092 Puno, M.R., Lima, C.D. Structural basis for RNA surveillance by the human nuclear exosome targeting (NEXT) complex. Cell 185, 2132-2147 (2022) doi: 10.1016/j.cell.2022.04.016 Das, M., Zattas, D., Zinder, J.C., Wasmuth, E.V., Henri, J., Lima, C.D. Substrate discrimination and quality control require each catalytic activity of TRAMP and the nuclear RNA exosome. Proc Natl Acad Sci U S A. 118:e2024846118. (2021) [PMID: 33782132] Hann, Z.S., Ji, C., Olsen, S.K., Lu, X., Lux, M.C., Tan, D.S., Lima, C.D.Structural basis for adenylation and thioester bond formation in the ubiquitin E1. Proc Natl Acad Sci U S A. 116, 15475-15484. (2019) [PMID: 31235585] Weick, E.M., Puno, M.R., Januszyk, K., Zinder, J.C., DiMattia, M.A., Lima, C.D. Helicase-Dependent RNA Decay Illuminated by a Cryo-EM Structure of a Human Nuclear RNA Exosome-MTR4 Complex. Cell 173, 1663-1677 (2018) [PMID: 29906447] Wasmuth, E.V., Zinder, J.C., Zattas, D., Das, M., and Lima, C.D. Structure and reconstitution of yeast Mpp6-nuclear exosome complexes reveals that Mpp6 stimulates RNA decay and recruits the Mtr4 helicase. Elife Jul 25;6. pii: e29062. doi: 10.7554/eLife.29062 (2017) [PMID: 28742025] Streich FC Jr, Lima CD. Capturing a substrate in an activated RING E3/E2-SUMO complex. Nature 536, 304-308 (2016) [PMID: 27509863] Zinder, J.C., Wasmuth, E.V., and Lima, C.D. Nuclear RNA exosome at 3.1 A reveals substrate specificities, RNA paths, and allosteric inhibition of Rrp44. Mol Cell 64, 734-735. (2016) [PMID: 27818140] Cappadocia, L., Pichler, A., and Lima, C.D. Structural basis for catalytic activation by the human ZNF451 SUMO E3 ligase. Nat Struct Mol Biol 22, 968-975. (2015) [PMID: 26524494] Bekes, M., van der Heden van Noort, G.J., Ekkebus, R., Ovaa, H., Huang, T.T., and Lima, C.D. Recognition of Lys48-linked di-ubiquitin and deubiquitinating activities of the SARS coronavirus papain-like protease. Mol Cell 62, 572-585. (2016) [PMID: 27203180] Wasmuth EV, Januszyk K, Lima CD. Structure of an Rrp6-RNA exosome complex bound to poly(A) RNA. Nature 511, 435-439 (2014) [PMID: 25043052] Armstrong AA, Mohideen F, Lima CD. Recognition of SUMO-modified PCNA requires tandem receptor motifs in Srs2. Nature 483, 59-63 (2012) [PMID: 22382979] Review Articles Weick, E.M. and Lima, C.D. RNA helicases are hubs that orchestrate exosome-dependent 3’-5’ decay. Curr Opin Struct Biol 67, 86-94 (2020) [PMID: 33147539] Puno, M.R., Weick, E.M., Das, M., Lima, C.D. Snapshot: The RNA exosome. Cell 179, 282-282 (2019) [PMID: 31539497] Cappadocia, L., Lima, C.D. Ubiquitin-like Protein Conjugation: Structures, Chemistry and Mechanism. Chem Rev. 118, 889-918 (2018) [PMID: 28234446] Book traversal links for The Christopher Lima Lab Previous Featured News Next Open Positions