The Molecular Microbiology Facility (MMF) is part of the Center for Microbes, Inflammation, and Cancer. The laboratory works with investigators to use next generation sequencing platforms to analyze the microbial composition of experimental samples obtained from mice or clinical samples obtained from patients. The laboratory performs DNA extraction and PCR amplification using bar-coded primers for bacterial 16S ribosomal RNA gene sequencing on the Illumina MiSeq platform. We amplify the V4-V5 region of the 16S rRNA gene and, depending on the sequencing depth required, can provide from several thousand to millions of sequences per sample. In addition to 16S sequencing, the lab performs shotgun metagenomic sequencing of fecal samples using the Illumina HiSeq platform. The laboratory works with investigators to analyze the microbiota of the small and large intestines, feces, saliva and oral mucosal surfaces and the urogenital tract.