The SKI Stem Cell Research Core stocks, characterizes, and distributes human pluripotent stem cell (hPSC) lines. We perform a variety of hPSC-related applications for the stem cell community at SKI and beyond. We are a part of the Starr Foundation-funded Tri-Institutional Stem Cell Initiative that provides shared stem cell expertise and resources among Memorial Sloan Kettering, Weill-Cornell, and Rockefeller University.
Techniques and services available at the facility include:
- Training in hPSC culture and genetic modification.
- hPSCs (hESCs/hiPSCs) banking.
- Gene editing in hPSCs using CRIPSR-based approaches including gene knock-out/ SNP knock-in and generation of gene corrected isogenic PSC lines.
- Generation of hPSC reporter lines by gene targeting
- iPSC derivation from primary fibroblasts/blood-derived (PBMCs) or urinary cells.
- Directed differentiation of hPSCs into various lineage-specific cell types.
- hPSC-related chemical screening.
- Sharing of specialized equipment.
- Custom services and collaborative projects with individual investigators or consortia
Additionally, we continually refine and adapt the newest technologies relevant to hPSC research including genetic manipulation, directed differentiation and reprogramming to offer state-of-the-art services to the Tri-Institutional community.
