Tobias Walther: Publications

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2022
2021
2020

Christiano R, Arlt H, Kabatnik S, Mejhert N, Lai ZW, Farese RV Jr, Walther TC. A Systematic Protein Turnover Map for Decoding Protein Degradation. Cell Rep. 2020 Nov 10;33(6):108378. doi: 10.1016/j.celrep.2020.108378. [PMID 33176155]

Chitraju C, Fischer AW, Farese RV Jr, Walther TC. Lipid Droplets in Brown Adipose Tissue Are Dispensable for Cold-Induced Thermogenesis. Cell Rep. 2020 Nov 3;33(5):108348. doi: 10.1016/j.celrep.2020.108348.

Becuwe M, Bond LM, Pinto AFM, Boland S, Mejhert N, Elliott SD, Cicconet M, Graham MM, Liu XN, Ilkayeva O, Saghatelian A, Walther TC, Farese RV. FIT2 is an acyl-coenzyme A diphosphatase crucial for endoplasmic reticulum homeostasis. J Cell Biol. 2020 Oct 5;219(10). doi: 10.1083/jcb.202006111.

Olarte MJ, Kim S, Sharp ME, Swanson JMJ, Farese RV Jr, Walther TC. Determinants of Endoplasmic Reticulum-to-Lipid Droplet Protein Targeting. Dev Cell. 2020 Aug 24;54(4):471-487.e7. doi: 10.1016/j.devcel.2020.07.001. Epub 2020 Jul 29. NIHMSID:NIHMS1617288.

Sui X, Wang K, Gluchowski NL, Elliott SD, Liao M, Walther TC, Farese RV Jr.Structure and catalytic mechanism of a human triacylglycerol-synthesis enzyme. Nature. 2020 May;581(7808):323-328. doi: 10.1038/s41586-020-2289-6. Epub 2020 May 13.

Petit CS, Lee JJ, Boland S, Swarup S, Christiano R, Lai ZW, Mejhert N, Elliott SD, McFall D, Haque S, Huang EJ, Bronson RT, Harper JW, Farese RV Jr, Walther TC. Inhibition of sphingolipid synthesis improves outcomes and survival in GARP mutant wobbler mice, a model of motor neuron degeneration. Natl Acad Sci U S A. 2020 May 12;117(19):10565-10574. doi: 10.1073/pnas.1913956117. Epub 2020 Apr 28.

Mejhert N, Kuruvilla L, Gabriel KR, Elliott SD, Guie MA, Wang H, Lai ZW, Lane EA, Christiano R, Danial NN, Farese RV Jr, Walther TC. Partitioning of MLX-Family Transcription Factors to Lipid Droplets Regulates Metabolic Gene Expression. Mol Cell. 2020 Mar 19;77(6):1251-1264.e9. doi: 10.1016/j.molcel.2020.01.014. Epub 2020 Feb 4.

2019

Chung J, Wu X, Lambert TJ, Lai ZW, Walther TC, Farese RV Jr. LDAF1 and Seipin Form a Lipid Droplet Assembly Complex. Dev Cell. 2019 Dec 2;51(5):551-563.e7. doi: 10.1016/j.devcel.2019.10.006. Epub 2019 Nov 7.

Walther TC, Farese RV Jr. Un-phased: Lipid Droplets Modulate the Bioavailability of Antibiotics. Dev Cell. 2019 Sep 9;50(5):530-532. doi: 10.1016/j.devcel.2019.08.009.

Vejnar CE, Abdel Messih M, Takacs CM, Yartseva V, Oikonomou P, Christiano R, Stoeckius M, Lau S, Lee MT, Beaudoin JD, Musaev D, Darwich-Codore H, Walther TC, Tavazoie S, Cifuentes D, Giraldez AJ. Genome wide analysis of 3’ UTR sequence elements and proteins regulating mRNA stability during maternal-to-zygotic transition in zebrafish. Genome Res. 2019 Jul;29(7):1100-1114. doi: 10.1101/gr.245159.118. Epub 2019 Jun 21.

Luscher A, Fröhlich F, Barisch C, Littlewood C, Metcalfe J, Leuba F, Palma A, Pirruccello M, Cesareni G, Stagi M, Walther TC, Soldati T, De Camilli P, Swan LE. Lowe syndrome-linked endocytic adaptors direct membrane cycling kinetics with OCRL in Dictyostelium discoideum. Mol Biol Cell. 2019 Aug 1;30(17):2268-2282. doi: 10.1091/mbc.E18-08-0510. Epub 2019 Jun 19.

Gluchowski NL, Gabriel KR, Chitraju C, Bronson RT, Mejhert N, Boland S, Wang K, Lai ZW, Farese RV Jr, Walther TC. Hepatocyte Deletion of Triglyceride-Synthesis Enzyme Acyl CoA: Diacylglycerol Acyltransferase 2 Reduces Steatosis Without Increasing Inflammation or Fibrosis in Mice. Hepatology. 2019 May 13. doi: 10.1002/hep.30765. [Epub ahead of print]

Chitraju C, Walther TC, Farese RV Jr. The triglyceride synthesis enzymes DGAT1 and DGAT2 have distinct and overlapping functions in adipocytes. J Lipid Res. 2019 Jun;60(6):1112-1120. doi: 10.1194/jlr.M093112. Epub 2019 Apr 1.

Piccolis M, Bond LM, Kampmann M, Pulimeno P, Chitraju C, Jayson CBK, Vaites LP, Boland S, Lai ZW, Gabriel KR, Elliott SD, Paulo JA, Harper JW, Weissman JS, Walther TC, Farese RV Jr. Probing the Global Cellular Responses to Lipotoxicity Caused by Saturated Fatty Acids. Mol Cell. 2019 Apr 4;74(1):32-44.e8. doi: 10.1016/j.molcel.2019.01.036. Epub 2019 Mar 4.

Fanning S, Haque A, Imberdis T, Baru V, Barrasa MI, Nuber S, Termine D, Ramalingam N, Ho GPH, Noble T, Sandoe J, Lou Y, Landgraf D, Freyzon Y, Newby G, Soldner F, Terry-Kantor E, Kim TE, Hofbauer HF, Becuwe M, Jaenisch R, Pincus D, Clish CB, Walther TC, Farese RV Jr, Srinivasan S, Welte MA, Kohlwein SD, Dettmer U, Lindquist S, Selkoe D. Lipidomic Analysis of α-Synuclein Neurotoxicity Identifies Stearoyl CoA Desaturase as a Target for Parkinson Treatment. Mol Cell. 2019 Mar 7;73(5):1001-1014.e8. doi: 10.1016/j.molcel.2018.11.028. Epub 2018 Dec 4.

2018

Sui X, Arlt H, Brock KP, Lai ZW, DiMaio F, Marks DS, Liao M, Farese RV Jr, Walther TC. Cryo-electron microscopy structure of the lipid droplet-formation protein seipin. Cell Biol. 2018 Dec 3;217(12):4080-4091. doi: 10.1083/jcb.201809067. Epub 2018 Oct 16.

Nguyen AD, Nguyen TA, Singh RK, Eberlé D, Zhang J, Abate JP, Robles A, Koliwad S, Huang EJ, Maxfield FR, Walther TC, Farese RV Jr. Progranulin in the hematopoietic compartment protects mice from atherosclerosis. Atherosclerosis. 2018 Aug 30;277:145-154. doi: 10.1016/j.atherosclerosis.2018.08.042. [Epub ahead of print]

Jayson CBK, Arlt H, Fischer AW, Lai ZW, Farese RV Jr, Walther TC. Rab18 is not necessary for lipid droplet biogenesis or turnover in human mammary carcinoma cells. Mol Biol Cell. 2018 Aug 15;29(17):2045-2054. doi: 10.1091/mbc.E18-05-0282. Epub 2018 Jun 27.

Nguyen AD, Nguyen TA, Zhang J, Devireddy S, Zhou P, Karydas AM, Xu X, Miller BL, Rigo F, Ferguson SM, Huang EJ, Walther TC, Farese RV Jr. Murine knockin model for progranulin-deficient frontotemporal dementia with nonsense-mediated mRNA decay. Proc Natl Acad Sci U S A. 2018 Mar 20;115(12): E2849-E2858. doi: 10.1073/pnas.1722344115. Epub 2018 Mar 6.

Li Z, Lai ZW, Christiano R, Gazos-Lopes F, Walther TC, Farese RV Jr. Global Analyses of Selective Insulin Resistance in Hepatocytes Caused by Palmitate Lipotoxicity.V. Mol Cell Proteomics. 2018 May;17(5):836-849. doi: 10.1074/mcp.RA117.000560. Epub 2018 Feb 5.

Prévost C, Sharp ME, Kory N, Lin Q, Voth GA, Farese RV Jr., Walther TC. Mechanism and Determinants of Amphipathic Helix-Containing Protein Targeting to Lipid Droplets. Dev Cell. 2018 Jan 8;44(1):73-86.e4. doi: 10.1016/j.devcel.2017.12.011. Epub 2018 Jan 8.

Inloes JM, Kiosses WB, Wang H, Walther TC, Farese RV Jr., Cravatt BF. Functional Contribution of the Spastic Paraplegia-Related Triglyceride Hydrolase DDHD2 to the Formation and Content of Lipid Droplets. Biochemistry. 2018 Feb 6;57(5):827-838. doi: 10.1021/acs. biochem.7b01028. Epub 2017 Dec 26.

Acosta-Alvear D, Karagöz GE, Fröhlich F, Li H, Walther TC, Walter P. The unfolded protein response and endoplasmic reticulum protein targeting machineries converge on the stress sensor IRE1. Elife. 2018 Dec 24;7. pii: e43036. doi: 10.7554/eLife.43036.

Krahmer N, Najafi B, Schueder F, Quagliarini F, Steger M, Seitz S, Kasper R, Salinas F, Cox J, Uhlenhaut NH, Walther TC, Jungmann R, Zeigerer A, Borner GHH, Mann M. Organellar Proteomics and Phospho-Proteomics Reveal Subcellular Reorganization in Diet-Induced Hepatic Steatosis. Dev Cell. 2018 Oct 22;47(2):205-221.e7. doi: 10.1016/j.devcel.2018.09.017.

Eikrem Ø, Walther TC, Flatberg A, Beisvag V, Strauss P, Farstad M, Beisland C, Koch E, Mueller TF, Marti HP. Fine needle aspirates of kidneys: a promising tool for RNA sequencing in native and transplanted kidneys. BMC Nephrol. 2018 Sep 5;19(1):221. doi: 10.1186/s12882-018-1012-4.

Goldberg IJ, Reue K, Abumrad NA, Bickel PE, Cohen S, Fisher EA, Galis ZS, Granneman JG, Lewandowski ED, Murphy R, Olive M, Schaffer JE, Schwartz-Longacre L, Shulman GI, Walther TC, Chen J. Deciphering the Role of Lipid Droplets in Cardiovascular Disease: A Report From the 2017 National Heart, Lung, and Blood Institute Workshop. Circulation. 2018 Jul 17;138(3):305-315.doi:10.1161/CIRCULATIONAHA.118.033704. Review.

2017

Inloes JM, Kiosses WB, Wang H, Walther TC, Farese RV Jr, Cravatt BF. Functional Contribution of the Spastic Paraplegia-Related Triglyceride Hydrolase DDHD2 to the Formation and Content of Lipid Droplets. Biochemistry. 2017 Dec 26. doi: 10.1021/acs.biochem.7b01028.

Sha SJ, Miller ZA, Min SW, Zhou Y, Brown J, Mitic LL, Karydas A, Koestler M, Tsai R, Corbetta-Rastelli C, Lin S, Hare E, Fields S, Fleischmann KE, Powers R, Fitch R, Martens LH, Shamloo M, Fagan AM, Farese RV Jr, Pearlman R, Seeley W, Miller BL, Gan L, Boxer AL. An 8-week, open-label, dose-finding study of nimodipine for the treatment of progranulin insufficiency from GRN gene mutations. Alzheimers Dement (N Y). 2017 Sep 12;3(4):507-512. doi: 10.1016/j.trci.2017.08.002. eCollection 2017 Nov.

Walther TC, Chung J, Farese RV Jr. Lipid Droplet Biogenesis. Annu Rev Cell Dev Biol. 2017 Oct 6;33:491-510. doi: 10.1146/annurev-cellbio-100616-060608. Epub 2017 Aug 9.

Chitraju C, Mejhert N, Haas JT, Diaz-Ramirez LG, Grueter CA, Imbriglio JE, Pinto S, Koliwad SK, Walther TC, Farese RV Jr. Triglyceride Synthesis by DGAT1 Protects Adipocytes from Lipid-Induced ER Stress during Lipolysis. Cell Metab. 2017 Aug 1;26(2):407-418.e3. doi: 10.1016/j.cmet.2017.07.012.

Christiano R, Kolev NG, Shi H, Ullu E, Walther TC, Tschudi C. The proteome and transcriptome of the infectious metacyclic form of Trypanosoma brucei define quiescent cells primed for mammalian invasion. Mol Microbiol. 2017 Oct;106(1):74-92. doi: 10.1111/mmi.13754. Epub 2017 Aug 4.

Krabbe G, Minami SS, Etchegaray JI, Taneja P, Djukic B, Davalos D, Le D, Lo I, Zhan L, Reichert MC, Sayed F, Merlini M, Ward ME, Perry DC, Lee SE, Sias A, Parkhurst CN, Gan WB, Akassoglou K, Miller BL, Farese RV Jr, Gan L. Microglial NFκB-TNFα hyperactivation induces obsessive-compulsive behavior in mouse models of progranulin-deficient frontotemporal dementia. Proc Natl Acad Sci U S A. 2017 May 9;114(19):5029-5034. doi: 10.1073/pnas.1700477114. Epub 2017 Apr 24.

Gluchowski NL, Becuwe M, Walther TC, Farese RV Jr. Lipid droplets and liver disease: from basic biology to clinical implications. Nat Rev Gastroenterol Hepatol. 2017 Jun;14(6):343-355. doi: 10.1038/nrgastro.2017.32. Epub 2017 Apr 21. Review.

Gluchowski NL, Chitraju C, Picoraro JA, Mejhert N, Pinto S, Xin W, Kamin DS, Winter HS, Chung WK, Walther TC, Farese RV Jr. Identification and characterization of a novel DGAT1 missense mutation associated with congenital diarrhea. J Lipid Res. 2017 Jun;58(6):1230-1237. doi: 10.1194/jlr.P075119. Epub 2017 Apr 3.

Kory N, Grond S, Kamat SS, Li Z, Krahmer N, Chitraju C, Zhou P, Fröhlich F, Semova I, Ejsing C, Zechner R, Cravatt BF, Farese RV Jr, Walther TC. Mice lacking lipid droplet-associated hydrolase, a gene linked to human prostate cancer, have normal cholesterol ester metabolism. J Lipid Res. 2017 Jan;58(1):226-235. doi: 10.1194/jlr.M072538. Epub 2016 Nov 11.

2016

Fröhlich F, Olson DK, Christiano R, Farese RV Jr, Walther TC. Proteomic and phosphoproteomic analyses of yeast reveal the global cellular response to sphingolipid depletion. Proteomics. 2016 Nov;16(21):2759-2763. doi: 10.1002/pmic.201600269. [PubMed ]PMID: 27717283]

Baskin JM, Wu X, Christiano R, Oh MS, Schauder CM, Gazzerro E, Messa M, Baldassari S, Assereto S, Biancheri R, Zara F, Minetti C, Raimondi A, Simons M, Walther TC, Reinisch KM, De Camilli P. The leukodystrophy protein FAM126A (hyccin) regulates PtdIns(4)P synthesis at the plasma membrane. Nat Cell Biol. 2016 Jan;18(1):132-8. doi: 10.1038/ncb3271. PubMed Central PMCID: PMC4689616.

Olson DK, Fröhlich F, Farese RV Jr, Walther TC. Taming the sphinx: Mechanisms of cellular sphingolipid homeostasis. Biochim Biophys Acta. 2016 Aug;1861(8 Pt B):784-92. doi: 10.1016/j.bbalip.2015.12.021. Review. PubMed Central PMCID: PMC4907851.

Kory N, Farese RV Jr, Walther TC. Targeting Fat: Mechanisms of Protein Localization to Lipid Droplets. Trends Cell Biol. 2016 Jul;26(7):535-46. doi: 10.1016/j.tcb.2016.02.007. Review. PubMed Central PMCID: PMC4976449.

Wang H, Becuwe M, Housden BE, Chitraju C, Porras AJ, Graham MM, Liu XN, Thiam AR, Savage DB, Agarwal AK, Garg A, Olarte MJ, Lin Q, Fröhlich F, Hannibal-Bach HK, Upadhyayula S, Perrimon N, Kirchhausen T, Ejsing CS, Walther TC, Farese RV. Seipin is required for converting nascent to mature lipid droplets. Elife. 2016 Aug 26;5. pii: e16582. doi: 10.7554/eLife.16582. PubMed Central PMCID: PMC5035145.

Farese RV Jr, Walther TC. Lipid droplets go nuclear. J Cell Biol. 2016 Jan 4;212(1):7-8. doi: 10.1083/jcb.201512056. PubMed Central PMCID: PMC4700486.

Lui H, Zhang J, Makinson SR, Cahill MK, Kelley KW, Huang HY, Shang Y, Oldham MC, Martens LH, Gao F, Coppola G, Sloan SA, Hsieh CL, Kim CC, Bigio EH, Weintraub S, Mesulam MM, Rademakers R, Mackenzie IR, Seeley WW, Karydas A, Miller BL, Borroni B, Ghidoni R, Farese RV Jr, Paz JT, Barres BA, Huang EJ. Progranulin Deficiency Promotes Circuit-Specific Synaptic Pruning by Microglia via Complement Activation. Cell. 2016 May 5;165(4):921-35. doi: 10.1016/j.cell.2016.04.001. PubMed Central PMCID: PMC4860138.

Agarwal AK, Tunison K, Dalal JS, Yen CL, Farese RV Jr, Horton JD, Garg A.Mogat1 deletion does not ameliorate hepatic steatosis in lipodystrophic (Agpat2-/-) or obese (ob/ob) mice. J Lipid Res. 2016 Apr;57(4):616-30. doi: 10.1194/jlr.M065896. PubMed Central PMCID: PMC4808770.

2015
2014

Bazzini AA, Johnstone TG, Christiano R, Mackowiak SD, Obermayer B, Fleming ES, Vejnar CE, Lee MT, Rajewsky N, Walther TC, Giraldez AJ. Identification of small ORFs in vertebrates using ribosome footprinting and evolutionary conservation. EMBO J. 2014;33(9):981-93. doi: 10.1002/embj.201488411. PMCID: PMC4193932.

Camus G, Schweiger M, Herker E, Harris C, Kondratowicz AS, Tsou CL, Farese RV, Jr., Herath K, Previs SF, Roddy TP, Pinto S, Zechner R, Ott M. The hepatitis C virus core protein inhibits adipose triglyceride lipase (ATGL)-mediated lipid mobilization and enhances the ATGL interaction with comparative gene identification 58 (CGI-58) and lipid droplets. J Biol Chem. 2014;289(52):35770-80. doi: 10.1074/jbc.M114.587816. PMCID: PMC4276846.

Christiano R, Nagaraj N, Frohlich F, Walther TC. Global proteome turnover analyses of the Yeasts S. cerevisiae and S. pombe. Cell Rep. 2014;9(5):1959-65. doi: 10.1016/j.celrep.2014.10.065. PMCID: PMC4526151.

Currie E, Guo X, Christiano R, Chitraju C, Kory N, Harrison K, Haas J, Walther TC, Farese RV, Jr. High confidence proteomic analysis of yeast LDs identifies additional droplet proteins and reveals connections to dolichol synthesis and sterol acetylation. J Lipid Res. 2014;55(7):1465-77. doi: 10.1194/jlr.M050229. PMCID: PMC4076087.

Frohlich F, Christiano R, Olson DK, Alcazar-Roman A, DeCamilli P, Walther TC. A role for eisosomes in maintenance of plasma membrane phosphoinositide levels. Mol Biol Cell. 2014;25(18):2797-806. doi: 10.1091/mbc.E13-11-0639. PMCID: PMC4161514.

Fu D, Yu Y, Folick A, Currie E, Farese RV, Jr., Tsai TH, Xie XS, Wang MC. In vivo metabolic fingerprinting of neutral lipids with hyperspectral stimulated Raman scattering microscopy. J Am Chem Soc. 2014;136(24):8820-8. doi: 10.1021/ja504199s. PMCID: PMC4073829.

Harrison MS, Hung CS, Liu TT, Christiano R, Walther TC, Burd CG. A mechanism for retromer endosomal coat complex assembly with cargo. Proc Natl Acad Sci U S A. 2014;111(1):267-72. doi: 10.1073/pnas.1316482111. PMCID: PMC3890810.

Liu L, Trent CM, Fang X, Son NH, Jiang H, Blaner WS, Hu Y, Yin YX, Farese RV, Jr., Homma S, Turnbull AV, Eriksson JW, Hu SL, Ginsberg HN, Huang LS, Goldberg IJ. Cardiomyocyte-specific loss of diacylglycerol acyltransferase 1 (DGAT1) reproduces the abnormalities in lipids found in severe heart failure. J Biol Chem. 2014;289(43):29881-91. doi: 10.1074/jbc.M114.601864. PMCID: PMC4207999.

Minami SS, Min SW, Krabbe G, Wang C, Zhou Y, Asgarov R, Li Y, Martens LH, Elia LP, Ward ME, Mucke L, Farese RV, Jr., Gan L. Progranulin protects against amyloid beta deposition and toxicity in Alzheimer’s disease mouse models. Nat Med. 2014;20(10):1157-64. doi: 10.1038/nm.3672. PMCID: PMC4196723.

Mul JD, Begg DP, Haller AM, Pressler JW, Sorrell J, Woods SC, Farese RV, Jr., Seeley RJ, Sandoval DA. MGAT2 deficiency and vertical sleeve gastrectomy have independent metabolic effects in the mouse.Am J Physiol Endocrinol Metab. 2014;307(11):E1065-72. doi: 10.1152/ajpendo.00376.2014. PMCID: PMC4254982.

Payne F, Lim K, Girousse A, Brown RJ, Kory N, Robbins A, Xue Y, Sleigh A, Cochran E, Adams C, Dev Borman A, Russel-Jones D, Gorden P, Semple RK, Saudek V, O’Rahilly S, Walther TC, Barroso I, Savage DB. Mutations disrupting the Kennedy phosphatidylcholine pathway in humans with congenital lipodystrophy and fatty liver disease. Proc Natl Acad Sci U S A. 2014;111(24):8901-6. doi: 10.1073/pnas.1408523111. PMCID: PMC4066527.

Ward ME, Taubes A, Chen R, Miller BL, Sephton CF, Gelfand JM, Minami S, Boscardin J, Martens LH, Seeley WW, Yu G, Herz J, Filiano AJ, Arrant AE, Roberson ED, Kraft TW, Farese RV, Jr., Green A, Gan L. Early retinal neurodegeneration and impaired Ran-mediated nuclear import of TDP-43 in progranulin-deficient FTLD. J Exp Med. 2014;211(10):1937-45. doi: 10.1084/jem.20140214. PMCID: PMC4172214.

Wilfling F, Thiam AR, Olarte MJ, Wang J, Beck R, Gould TJ, Allgeyer ES, Pincet F, Bewersdorf J, Farese RV, Jr., Walther TC. Arf1/COPI machinery acts directly on lipid droplets and enables their connection to the ER for protein targeting. Elife. 2014;3:e01607. doi: 10.7554/eLife.01607. PMCID: PMC3913038.

Wilfling F, Haas JT, Walther TC, Farese RV, Jr. Lipid droplet biogenesis. Curr Opin Cell Biol. 2014;29:39-45. doi: 10.1016/j.ceb.2014.03.008. PMCID: PMC4526149.

2013

Camus G, Herker E, Modi AA, Haas JT, Ramage HR, Farese RV, Jr., Ott M. Diacylglycerol acyltransferase-1 localizes hepatitis C virus NS5A protein to lipid droplets and enhances NS5A interaction with the viral capsid core. J Biol Chem. 2013;288(14):9915-23. doi: 10.1074/jbc.M112.434910. PMCID: PMC3617291.

Chen X, Chang J, Deng Q, Xu J, Nguyen TA, Martens LH, Cenik B, Taylor G, Hudson KF, Chung J, Yu K, Yu P, Herz J, Farese RV, Jr., Kukar T, Tansey MG. Progranulin does not bind tumor necrosis factor (TNF) receptors and is not a direct regulator of TNF-dependent signaling or bioactivity in immune or neuronal cells. J Neurosci. 2013;33(21):9202-13. doi: 10.1523/JNEUROSCI.5336-12.2013. PMCID: PMC3707136.

Currie E, Schulze A, Zechner R, Walther TC, Farese RV, Jr. Cellular fatty acid metabolism and cancer. Cell Metab. 2013;18(2):153-61. doi: 10.1016/j.cmet.2013.05.017. PMCID: PMC3742569.

Filiano AJ, Martens LH, Young AH, Warmus BA, Zhou P, Diaz-Ramirez G, Jiao J, Zhang Z, Huang EJ, Gao FB, Farese RV, Jr., Roberson ED. issociation of frontotemporal dementia-related deficits and neuroinflammation in progranulin haploinsufficient mice. J Neurosci. 2013;33(12):5352-61. doi: 10.1523/JNEUROSCI.6103-11.2013. PMCID: PMC3740510.

Frohlich F, Christiano R, Walther TC. Native SILAC: metabolic labeling of proteins in prototroph microorganisms based on lysine synthesis regulation. Mol Cell Proteomics. 2013;12(7):1995-2005. doi: 10.1074/mcp.M112.025742. PMCID: PMC3708181.

Klemm RW, Norton JP, Cole RA, Li CS, Park SH, Crane MM, Li L, Jin D, Boye-Doe A, Liu TY, Shibata Y, Lu H, Rapoport TA, Farese RV, Jr., Blackstone C, Guo Y, Mak HY. A conserved role for atlastin GTPases in regulating lipid droplet size. Cell Rep. 2013;3(5):1465-75. doi: 10.1016/j.celrep.2013.04.015. PMCID: PMC3742324.

Krahmer N, Hilger M, Kory N, Wilfling F, Stoehr G, Mann M, Farese RV, Jr., Walther TC. Protein correlation profiles identify lipid droplet proteins with high confidence. Mol Cell Proteomics. 2013;12(5):1115-26. doi: 10.1074/mcp.M112.020230. PubMed PMID: 23319140; PMCID: PMC3650325.
Krahmer N, Farese RV, Jr., Walther TC. Balancing the fat: lipid droplets and human disease. EMBO Mol Med. 2013;5(7):905-15. doi: 10.1002/emmm.201100671. PubMed PMID: 23740690; PMCID: PMC3721468.

Nguyen AD, Nguyen TA, Cenik B, Yu G, Herz J, Walther TC, Davidson WS, Farese RV, Jr. Secreted progranulin is a homodimer and is not a component of high density lipoproteins (HDL). J Biol Chem. 2013;288(12):8627-35. doi: 10.1074/jbc.M112.441949. PubMed PMID: 23364791; PMCID: PMC3605681.
Nguyen AD, Nguyen TA, Martens LH, Mitic LL, Farese RV, Jr. Progranulin: at the interface of neurodegenerative and metabolic diseases. Trends Endocrinol Metab. 2013;24(12):597-606. doi: 10.1016/j.tem.2013.08.003. PubMed PMID: 24035620; PMCID: PMC3842380.

Shimazu T, Hirschey MD, Newman J, He W, Shirakawa K, Le Moan N, Grueter CA, Lim H, Saunders LR, Stevens RD, Newgard CB, Farese RV, Jr., de Cabo R, Ulrich S, Akassoglou K, Verdin E. Suppression of oxidative stress by beta-hydroxybutyrate, an endogenous histone deacetylase inhibitor. Science. 2013;339(6116):211-4. doi: 10.1126/science.1227166. PubMed PMID: 23223453; PMCID: PMC3735349.

Thiam AR, Antonny B, Wang J, Delacotte J, Wilfling F, Walther TC, Beck R, Rothman JE, Pincet F. COPI buds 60-nm lipid droplets from reconstituted water-phospholipid-triacylglyceride interfaces, suggesting a tension clamp function. Proc Natl Acad Sci U S A. 2013;110(33):13244-9. doi: 10.1073/pnas.1307685110. PubMed PMID: 23901109; PMCID: PMC3746913.

Thiam AR, Farese RV, Jr., Walther TC. The biophysics and cell biology of lipid droplets. Nat Rev Mol Cell Biol. 2013;14(12):775-86. doi: 10.1038/nrm3699. PubMed PMID: 24220094; PMCID: PMC4526153.

Wilfling F, Wang H, Haas JT, Krahmer N, Gould TJ, Uchida A, Cheng JX, Graham M, Christiano R, Frohlich F, Liu X, Buhman KK, Coleman RA, Bewersdorf J, Farese RV, Jr., Walther TC. Triacylglycerol synthesis enzymes mediate lipid droplet growth by relocalizing from the ER to lipid droplets. Dev Cell. 2013;24(4):384-99. doi: 10.1016/j.devcel.2013.01.013. PubMed PMID: 23415954; PMCID: PMC3727400.

Zhang Z, Almeida S, Lu Y, Nishimura AL, Peng L, Sun D, Wu B, Karydas AM, Tartaglia MC, Fong JC, Miller BL, Farese RV, Jr., Moore MJ, Shaw CE, Gao FB. Downregulation of microRNA-9 in iPSC-derived neurons of FTD/ALS patients with TDP-43 mutations. PLoS One. 2013;8(10):e76055. doi: 10.1371/journal.pone.0076055. PubMed PMID: 24143176; PMCID: PMC3797144.

2012

Almeida S, Zhang Z, Coppola G, Mao W, Futai K, Karydas A, Geschwind MD, Tartaglia MC, Gao F, Gianni D, Sena-Esteves M, Geschwind DH, Miller BL, Farese RV, Jr., Gao FB. Induced pluripotent stem cell models of progranulin-deficient frontotemporal dementia uncover specific reversible neuronal defects. Cell Rep. 2012;2(4):789-98. doi: 10.1016/j.celrep.2012.09.007. PubMed PMID: 23063362; PMCID: PMC3532907.

Armakola M, Higgins MJ, Figley MD, Barmada SJ, Scarborough EA, Diaz Z, Fang X, Shorter J, Krogan NJ, Finkbeiner S, Farese RV, Jr., Gitler AD. Inhibition of RNA lariat debranching enzyme suppresses TDP-43 toxicity in ALS disease models. Nat Genet. 2012;44(12):1302-9. doi: 10.1038/ng.2434. PubMed PMID: 23104007; PMCID: PMC3510335.

Eichmann TO, Kumari M, Haas JT, Farese RV, Jr., Zimmermann R, Lass A, Zechner R. Studies on the substrate and stereo/regioselectivity of adipose triglyceride lipase, hormone-sensitive lipase, and diacylglycerol-O-acyltransferases. J Biol Chem. 2012;287(49):41446-57. doi: 10.1074/jbc.M112.400416. PubMed PMID: 23066022; PMCID: PMC3510842.

Haas JT, Winter HS, Lim E, Kirby A, Blumenstiel B, DeFelice M, Gabriel S, Jalas C, Branski D, Grueter CA, Toporovski MS, Walther TC, Daly MJ, Farese RV, Jr. DGAT1 mutation is linked to a congenital diarrheal disorder. J Clin Invest. 2012;122(12):4680-4. doi: 10.1172/JCI64873. PubMed PMID: 23114594; PMCID: PMC3533555.

Haas JT, Miao J, Chanda D, Wang Y, Zhao E, Haas ME, Hirschey M, Vaitheesvaran B, Farese RV, Jr., Kurland IJ, Graham M, Crooke R, Foufelle F, Biddinger SB. Hepatic insulin signaling is required for obesity-dependent expression of SREBP-1c mRNA but not for feeding-dependent expression. Cell Metab. 2012;15(6):873-84. doi: 10.1016/j.cmet.2012.05.002. PubMed PMID: 22682225; PMCID: PMC3383842.

Farese RV, Jr., Zechner R, Newgard CB, Walther TC. The problem of establishing relationships between hepatic steatosis and hepatic insulin resistance. Cell Metab. 2012;15(5):570-3. doi: 10.1016/j.cmet.2012.03.004. PubMed PMID: 22560209; PMCID: PMC3767424.

Martens LH, Zhang J, Barmada SJ, Zhou P, Kamiya S, Sun B, Min SW, Gan L, Finkbeiner S, Huang EJ, Farese RV, Jr. Progranulin deficiency promotes neuroinflammation and neuron loss following toxin-induced injury. J Clin Invest. 2012;122(11):3955-9. doi: 10.1172/JCI63113. PubMed PMID: 23041626; PMCID: PMC3484443.

Streeper RS, Grueter CA, Salomonis N, Cases S, Levin MC, Koliwad SK, Zhou P, Hirschey MD, Verdin E, Farese RV, Jr. Deficiency of the lipid synthesis enzyme, DGAT1, extends longevity in mice. Aging (Albany NY). 2012;4(1):13-27. PubMed PMID: 22291164; PMCID: PMC3292902.

Tschop MH, Speakman JR, Arch JR, Auwerx J, Bruning JC, Chan L, Eckel RH, Farese RV, Jr., Galgani JE, Hambly C, Herman MA, Horvath TL, Kahn BB, Kozma SC, Maratos-Flier E, Muller TD, Munzberg H, Pfluger PT, Plum L, Reitman ML, Rahmouni K, Shulman GI, Thomas G, Kahn CR, Ravussin E. A guide to analysis of mouse energy metabolism. Nat Methods. 2012;9(1):57-63. doi: 10.1038/nmeth.1806. PubMed PMID: 22205519; PMCID: PMC3654855.

Walther TC, Farese RV, Jr. Lipid droplets and cellular lipid metabolism. Annu Rev Biochem. 2012;81:687-714. doi: 10.1146/annurev-biochem-061009-102430. PubMed PMID: 22524315; PMCID: PMC3767414.

Xu N, Zhang SO, Cole RA, McKinney SA, Guo F, Haas JT, Bobba S, Farese RV, Jr., Mak HY. The FATP1-DGAT2 complex facilitates lipid droplet expansion at the ER-lipid droplet interface. J Cell Biol. 2012;198(5):895-911. doi: 10.1083/jcb.201201139. PubMed PMID: 22927462; PMCID: PMC3432760.

Zhang J, Kelley KL, Marshall SM, Davis MA, Wilson MD, Sawyer JK, Farese RV, Jr., Brown JM, Rudel LL. Tissue-specific knockouts of ACAT2 reveal that intestinal depletion is sufficient to prevent diet-induced cholesterol accumulation in the liver and blood. J Lipid Res. 2012;53(6):1144-52. doi: 10.1194/jlr.M024356. PubMed PMID: 22460046; PMCID: PMC3351821.

2011

Bewersdorf J, Farese RV, Jr., Walther TC. A new way to look at fat. Nat Methods. 2011;8(2):132-3. doi: 10.1038/nmeth0211-132.

Cenik B, Sephton CF, Dewey CM, Xian X, Wei S, Yu K, Niu W, Coppola G, Coughlin SE, Lee SE, Dries DR, Almeida S, Geschwind DH, Gao FB, Miller BL, Farese RV, Jr., Posner BA, Yu G, Herz J. Suberoylanilide hydroxamic acid (vorinostat) up-regulates progranulin transcription: rational therapeutic approach to frontotemporal dementia. J Biol Chem. 2011;286(18):16101-8. doi: 10.1074/jbc.M110.193433. PMCID: PMC3091219. 

Fei W, Shui G, Zhang Y, Krahmer N, Ferguson C, Kapterian TS, Lin RC, Dawes IW, Brown AJ, Li P, Huang X, Parton RG, Wenk MR, Walther TC, Yang H. A role for phosphatidic acid in the formation of “supersized” lipid droplets. PLoS Genet. 2011;7(7):e1002201. doi: 10.1371/journal.pgen.1002201. PMCID: PMC3145623. 

Harris CA, Haas JT, Streeper RS, Stone SJ, Kumari M, Yang K, Han X, Brownell N, Gross RW, Zechner R, Farese RV, Jr. DGAT enzymes are required for triacylglycerol synthesis and lipid droplets in adipocytes. J Lipid Res. 2011;52(4):657-67. doi: 10.1194/jlr.M013003. PMCID: PMC3284159. 

Harris C, Herker E, Farese RV, Jr., Ott M. Hepatitis C virus core protein decreases lipid droplet turnover: a mechanism for core-induced steatosis. J Biol Chem. 2011;286(49):42615-25. doi: 10.1074/jbc.M111.285148. PMCID: PMC3234948. 

Hirschey MD, Shimazu T, Jing E, Grueter CA, Collins AM, Aouizerat B, Stancakova A, Goetzman E, Lam MM, Schwer B, Stevens RD, Muehlbauer MJ, Kakar S, Bass NM, Kuusisto J, Laakso M, Alt FW, Newgard CB, Farese RV, Jr., Kahn CR, Verdin E. SIRT3 deficiency and mitochondrial protein hyperacetylation accelerate the development of the metabolic syndrome. Mol Cell. 2011;44(2):177-90. doi: 10.1016/j.molcel.2011.07.019. PMCID: PMC3563434. 

Kao AW, Eisenhut RJ, Martens LH, Nakamura A, Huang A, Bagley JA, Zhou P, de Luis A, Neukomm LJ, Cabello J, Farese RV, Jr., Kenyon C. A neurodegenerative disease mutation that accelerates the clearance of apoptotic cells. Proc Natl Acad Sci U S A. 2011;108(11):4441-6. doi: 10.1073/pnas.1100650108. PMCID: PMC3060230. 

Karotki L, Huiskonen JT, Stefan CJ, Ziolkowska NE, Roth R, Surma MA, Krogan NJ, Emr SD, Heuser J, Grunewald K, Walther TC. Eisosome proteins assemble into a membrane scaffold. J Cell Biol. 2011;195(5):889-902. doi: 10.1083/jcb.201104040. PMCID: PMC3257569. 

Krahmer N, Guo Y, Wilfling F, Hilger M, Lingrell S, Heger K, Newman HW, Schmidt-Supprian M, Vance DE, Mann M, Farese RV, Jr., Walther TC. Phosphatidylcholine synthesis for lipid droplet expansion is mediated by localized activation of CTP:phosphocholine cytidylyltransferase. Cell Metab. 2011;14(4):504-15. doi: 10.1016/j.cmet.2011.07.013. PMCID: PMC3735358. 

Monetti M, Levin MC, Watt MJ, Hubbard BK, Newgard C, Farese RV, Sr., Hevener AL, Farese RV, Jr. Hepatic acyl-CoA:diacylglcyerol acyltransferase (DGAT) overexpression, diacylglycerol, and insulin sensitivity. Proc Natl Acad Sci U S A. 2011;108(34):E523; author reply E4. doi: 10.1073/pnas.1108505108. PMCID: PMC3161537. 

Rosen EY, Wexler EM, Versano R, Coppola G, Gao F, Winden KD, Oldham MC, Martens LH, Zhou P, Farese RV, Jr., Geschwind DH. Functional genomic analyses identify pathways dysregulated by progranulin deficiency, implicating Wnt signaling. Neuron. 2011;71(6):1030-42. doi: 10.1016/j.neuron.2011.07.021. PMCID: PMC3633414. 

Ziolkowska NE, Karotki L, Rehman M, Huiskonen JT, Walther TC. Eisosome-driven plasma membrane organization is mediated by BAR domains. Nat Struct Mol Biol. 2011;18(7):854-6. doi: 10.1038/nsmb.2080.

2010

Aguilar PS, Frohlich F, Rehman M, Shales M, Ulitsky I, Olivera-Couto A, Braberg H, Shamir R, Walter P, Mann M, Ejsing CS, Krogan NJ, Walther TC. A plasma-membrane E-MAP reveals links of the eisosome with sphingolipid metabolism and endosomal trafficking. Nat Struct Mol Biol. 2010;17(7):901-8. doi: 10.1038/nsmb.1829. PMCID: PMC3073498. 

Aguilar PS, Heiman MG, Walther TC, Engel A, Schwudke D, Gushwa N, Kurzchalia T, Walter P. Structure of sterol aliphatic chains affects yeast cell shape and cell fusion during mating. Proc Natl Acad Sci U S A. 2010;107(9):4170-5. doi: 10.1073/pnas.0914094107. [PMID: 20150508] ; PMCID: PMC2840153.
Herker E, Harris C, Hernandez C, Carpentier A, Kaehlcke K, Rosenberg AR, Farese RV, Jr., Ott M. Efficient hepatitis C virus particle formation requires diacylglycerol acyltransferase-1. Nat Med. 2010;16(11):1295-8. doi: 10.1038/nm.2238. PMCID: PMC3431199. 

Hirschey MD, Shimazu T, Goetzman E, Jing E, Schwer B, Lombard DB, Grueter CA, Harris C, Biddinger S, Ilkayeva OR, Stevens RD, Li Y, Saha AK, Ruderman NB, Bain JR, Newgard CB, Farese RV, Jr., Alt FW, Kahn CR, Verdin E. SIRT3 regulates mitochondrial fatty-acid oxidation by reversible enzyme deacetylation. Nature. 2010;464(7285):121-5. doi: 10.1038/nature08778. PMCID: PMC2841477.

Jiao J, Herl LD, Farese RV, Gao FB. MicroRNA-29b regulates the expression level of human progranulin, a secreted glycoprotein implicated in frontotemporal dementia. PLoS One. 2010;5(5):e10551. doi: 10.1371/journal.pone.0010551. PMCID: PMC2866661.

Koliwad SK, Streeper RS, Monetti M, Cornelissen I, Chan L, Terayama K, Naylor S, Rao M, Hubbard B, Farese RV, Jr. DGAT1-dependent triacylglycerol storage by macrophages protects mice from diet-induced insulin resistance and inflammation. J Clin Invest. 2010;120(3):756-67. doi: 10.1172/JCI36066. PMCID: PMC2827941. 

Walther TC. Keeping sphingolipid levels nORMal. Proc Natl Acad Sci U S A. 2010;107(13):5701-2. doi: 10.1073/pnas.1001503107. PMCID: PMC2851878. 

Walther TC, Mann M. Mass spectrometry-based proteomics in cell biology. J Cell Biol. 2010;190(4):491-500. doi: 10.1083/jcb.201004052.   PMCID: PMC2928005. 

Walther TC, Olsen JV, Mann M. Yeast expression proteomics by high-resolution mass spectrometry. Methods Enzymol. 2010;470:259-80. doi: 10.1016/S0076-6879(10)70011-2.

Complete Publications List

For older publications, please visit the complete publication lists of Dr. Robert Farese, Jr. and Dr. Tobias Walther.