Single-Cell Analysis Learning Enrichment (SCALE) Course at Memorial Sloan Kettering for NYC-Area Researchers: 5-Day Intensive Workshop on Analyzing Single-Cell RNA-Sequencing Data
March 25-29, 2024
Memorial Sloan Kettering Cancer Center
417 East 68th Street
New York, NY 10065
Note: this course will only be offered in person.
Memorial Sloan Kettering’s Single-Cell Analytics Innovation Lab, Epigenetics Research Innovation Lab, and Office of Scientific Education and Training are pleased to offer a 5-day in-person course for 24 NYC-area researchers on analyzing single-cell RNA-sequencing data. Researchers will learn about current state-of-the-art computational methods to analyze single-cell data under the mentorship of expert MSK faculty and those centrally involved in single-cell RNA-sequencing analyses.
The goal of this course is to increase participants’ fluency in single-cell RNA-sequencing analysis and to provide concrete tools to tackle the most pertinent open questions in their research fields. Participants will be equipped to apply these lessons on single-cell analysis to their own data to enable correct biological interpretations. Participants will learn about the latest technologies and best practices of experimental methods and effective data generation, state-of-the-art algorithms used in single-cell data analysis, and tools for relating their computational results to make novel discoveries in their biological field.
The course will include detailed instruction on algorithms, applications of the analyses, and hands-on analysis, culminating in a group project and presentation re-creating a publishable figure from real-world single-cell data. Participants will receive daily personalized support and guidance from multiple leaders in the field and will have opportunities to build community and network with the instructors, guest lecturers, and fellow participants.
March 25-29, 2024
Participants are expected to be on site each day from 8:30am-5:30pm. No lodging will be provided.
Each day will begin at 8:30am with a light breakfast. Lunch is on your own from 12:00pm-1:15pm, and afternoon coffee and sweets & fruit will be available at 3:00pm. There will be a morning Q&A session from 9:00am, and each day will end with a second Q&A session from 5:00-5:30pm.
Day 1: Introduction to Single-Cell Data
- Introduction to the course
- Overview of the development of genomics technologies
- Bulk RNA-seq introduction:
- Experimental design for scRNA-seq
- Genome/transcriptome alignment
- Data structure and loading data onto Python, QC
Day 2: Single-Cell Data Visualization
- Pre-processing, normalizing data, feature selection, dimensionality reduction
- Overview of single-cell RNA-sequencing and its impact over the years
- Graphical representation of data:
- Doublet detection
- Visualization and interpretation of data
Day 3: Cell Typing and Pseudotime Analysis
- Differential expression analysis:
- Gene set enrichment analysis
- Cell typing
- Milo; SPECTRA
- scRNA-seq data and computation to study cellular behavior in disease context
- Pseudotime analysis
- Data denoising and imputation
- Beyond pedestrian scRNA-seq: Enrichment experiments
Day 4: Multi-Sample Analysis and Batch Effect
- Loading multiple samples
- Detecting and correcting batch effect
- Novel computational tools for analysis of scRNA-seq, scATAC-seq, and beyond
- Multi-sample analysis continued
- Additional data wrangling
Day 5: Hands-On Analysis to Recreate a Published Figure
Class is divided into groups, and each group spends the day working on their project and presenting to the class and the instructors
MSK Lecturers and Organizers
Ronan Chaligne, PhD
Associate Lab Member, Single Cell Analytics Innovation Lab
Karuna Ganesh, MD, PhD
Assistant Member, Molecular Pharmacology Program
Richard Koche, PhD
Assistant Lab Member, Epigenetics Research Innovation Lab
Christina Leslie, PhD
Member, Computational and Systems Biology Program
Neeman Mohibullah, PhD
Associate Lab Member, Integrated Genomics Operation
Ushma Neill, PhD
Vice President, Scientific Education and Training
Dana Pe’er, PhD
Chair, Computational and Systems Biology Program
Roshan Sharma, PhD
Manager, Computational Biologist, Single Cell Analytics Innovation Lab
Thalyana Stathis, PhD
Associate Director, Office of Career and Professional Development
Course attendance will be capped at 24 participants.
Academic and industry researchers from all biomedical fields are welcomed and graduate students (who have passed their qualifying exam) & postdocs are encouraged to apply. Researchers from underrepresented racial and ethnic groups, and those with disabilities or from disadvantaged backgrounds, are also especially encouraged to apply. As no lodging will be provided, we will prioritize senior graduate students, postdocs, staff scientists, and junior faculty from local NYC-area universities and non-profit research institutions, and NYC-area industry scientists.
The course aims to educate wet-lab researchers to be able to independently analyze their own single-cell RNA-sequencing data in the future (they do not already need to have a dataset in hand). Participants are expected to already have programming fluency and be comfortable with basic data processing in R or Python. No time will be spent on the basics of Python, and the course will move at a fast pace; please do not register if you are not fluent in Python. However, participants with programming fluency in R will be brought up to speed in Python.
Participants are not expected to have had any previous exposure to single-cell data analysis tools. Pre-course content will situate participants with a basic introduction to Scanpy, a Python package designed for analysis of single-cell RNA-sequencing data, which hosts an annotated data structure (AnnData) aptly designed for handling such data.
Note: If you work at MSK, please do not apply to this course. Instead, look out for emails sent by the Office of Scientific Education and Training about applying for all of the courses that are offered internally.
- Academic/non-profit participants: $500
- Industry participants: $750
Note: You will be contacted regarding payment after you have been accepted into the course.
- February 18, 11:59PM EST: Deadline to apply; early applications are encouraged
- February 26: 24 participants will be notified; payment will be requested at this time. A waitlist will be maintained
- March 18: Deadline to complete pre-course survey; pre-course materials will be available upon survey completion
- March 25: Course begins
- March 29: Course ends
- April 5: Deadline to complete post-course survey
Applications are closed for Spring 2024. The application deadline was February 18, 11:59PM EST to be considered for a spot in the course.
If you have questions regarding future course offerings, please email Office of Scientific Education and Training at [email protected] with “SCALE course” in the subject line.
If you have any questions about the SCALE course, please email Office of Scientific Education and Training at [email protected] with “SCALE course” in the subject line.