The Nuclear Magnetic Resonance Core Facility supports MSK researchers by providing essential analytical services, access and education for analytical instrumentation and related shared scientific resources. Our main instrumentation includes high field nuclear magnetic resonance, mass spectrometry and optical spectroscopic instruments. Our primary focus concerns small molecule synthetic organic chemistry/drug development; however we support and welcome all MSK research that is within our capabilities. Through the use of our instruments, the facility affords investigators the ability to monitor chemical reactions, analysis for determination of molecular structure, chemical mechanisms, purity, stability, biological uptake and other properties of chemical compounds.
We currently have the following instrumentation:
- High Field NMR Spectroscopy: Five Bruker 500 and 600 MHz Spectrometers for liquids with BBO, TXO (13C&19F/1H), BBI, QNP (13C|31P|19F/1H), HRMAS (13C/1H), 1mm TXI and DCH (13C/1H) cryoprobe
- Mass Spectrometry: Eleven instruments with varying capabilities from Waters, Agilent, Thermo and SCIEX. Mass instruments include single quadrupole, triple quad and TOF. Capabilities include direct inject, LC-MS, exact mass, GC-MS/FID. Ionization is mainly ESI; APCI is available and the GC-MS instrument has both EI and CI capabilities. LC-MS capability include HPLC, UPLC, analytical and prep, automatic sample injectors, collectors and ELSD, PDA and UV-Vis detection.
- Spectroscopic instruments: Polarimetry with multi wavelength capability, UV-Vis and FTIR.
NMR is a powerful analytical technique, which is used to determine the number, location, and attachment of NMR active nuclei within molecules. The information obtained may be used to determine molecular structure, purity, reaction progress, kinetics, metabolic products and quantitation.
The NMR instruments in our facility routinely analyze small molecule proton, carbon, fluorine, and phosphorous nuclei of solvated compounds. Other nuclei detection are also available. Solids, peptides/proteins and large molecule may be sent to NYSBC.
Mass spectrometry determines the molecular mass of compounds and their fragments. Most of our instruments ionize via electrospray or APCI. Both techniques provide very gentle ionization of the molecule and often generate information about the base peak with little fragmentation, which is of primary interest for most chemists. The facility routinely analyzes compounds up to 6,000 AMU.
Send an email to the core head for more information. Generally first time requests need to submitted via iLab. Further submissions of samples entail submission of the appropriate request forms (available at the facility) along with the compounds of interest in appropriate containers. Please find out requirements first before submitting samples.