Services
shRNA cloning - Individual clone and pooled library
- RNA polymerase II-transcribed miR-E shRNA with high potency and specificity designed with the SpalshRNA algorithm.
- Options including transduction (lentiviral or retroviral), expression (constitutive or inducible), fluorescence reporter (GFP, dsRED etc), and selection marker (puromycin, hygromycin etc).
- Various target-focused pooled libraries including human and mouse druggable genome, epigenome, and kinome.
- Additional services including virus packaging and transduction.
sgRNA cloning - Individual clone and pooled library
- Options including fluorescence reporter (GFP, dsRED etc), and selection marker (puromycin, hygromycin etc).
- Full-genome libraries including GeCKO v1 (human), GeCKO v2 (human and mouse), Brie (mouse), Brunello (human), and SAM (human).
- Additional services including virus packaging and transduction.
Gene knock-in and knock-out cell line production
Pooled library screening - shRNA and sgRNA
- Assay development including transduction optimization.
- Screening execution, genomic DNA extraction, and PCR for next generation sequencing.
- Data analysis.
Arrayed library screening - siRNA and compound
- Human full-genome siRNA library (siGenome, Dharmacon).
- Bioactives and FDA-approved drug libraries, target focused inhibitor libraries against kinase and GPCR, and diversity libraries.
- Assay development and semi-automated screening in 384-well plate density format.
- Cell viability assay and biochemical assay with EnSpire multimode plate reader (PerkinElmer).
- High content cell imaging assay with IN Cell Analyzer 6000 laser-based confocal imaging platform (GE Healthcare) and Columbus image analysis system (PerkinElmer).