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Hong-fen Guo

Research Assistant/Specialist

Humanized monoclonal antibodies and single chain V-fragment (scFv) fusion proteins

The usage of monoclonal antibodies (MoAb) has recently been shown effective against a variety of human solid tumors. Monoclonal antibody treatment for cancer may cause side effects, some of which, though rare, can be very serious. We are humanizing mouse antibodies with proven clinical potential. We have focused our effort on humanized antibodies of the IgG1 and IgG4 subclass as well as their special glycoforms. We are also testing various forms of single chain V-fragment (scFv)-fusion proteins for therapeutic applications. We continue to test T cells or NK cells modified with scFv-chimeric immune receptors (CIR) as potential vehicles in tumor surveillance.

Neuroblastoma tumor cell line database

In using mammalian cell culture, researchers usually are more concerned about microbial contamination than cross-contamination between different cell lines (both intra- and inter-species). Authentication of cell lines are critical in assuring validity of the experiments and reproducibility of research. It is estimated that more than 20 percent of cell lines in the research community are misidentified or contaminated. Presently, cell line information is mainly provided by collections on private cell line databases. An STR (short tandem repeat) profile international reference standard for human cell lines was proposed by the leading cell banks from the American Type Culture Collection (ATCC). In the past decades, we have continued to establish neuroblastoma cell lines from fresh tumor samples or bone marrow from patients at the time of diagnosis or at the time of relapse. We are using STR typing methods, to authenticate all our neuroblastoma tumor cell lines in building a cell line database.