Mouse Genetics Core Facility (MGCF) at Memorial Sloan-Kettering Cancer Center (MSKCC) provides a range of technical support to facilitate the use of genetically engineered mice (GEM) for in vivo studies to dissect gene functions in complex biological processes that are of interest to investigators at MSKCC and collaborating institutions in the region. Expertise of MGCF encompasses molecular and cell biology to design and generate a variety of reagents for mouse genome engineering, embryo micromanipulation techniques to produce GEMs, and reproductive biology platform to maximize the utility of established GEM resources.
- Design and generation of a variety of molecular reagents for mouse genome engineering; such as transgene DNA, gene targeting components, and genome editing agents.
- Mouse embryonic stem (ES) cells; gene targeting by conventional homologous recombination methods and genome editing approaches, as well as ES cell line derivation and maintenance.
- Embryo micromanipulation and production of GEMs; via zygote micromanipulation for transgenesis and genome editing and ES cell injection into host embryos to generate ES-derived F0 mice.
- Primary molecular screening of GEMs.
- Cryopreservation of embryos and sperm for storage/archiving of GEM lines.
- Rederivation of cryopreserved embryos and in vitro fertilization (IVF) of cryopreserved sperm for strain recovery and importation.
- Provision of specialized mouse lines, ES cells and related molecular and cell biology reagents.
- Assistance and training in specialized procedures in reproductive biology.
- Consultation and discussion in in vivo genetic approaches.
For MSKCC investigators;
For more information, visit MGCF’s Intranet Site: