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HT-144: Human Melanoma Cell Line

SK1980-544

Description

HT-144 is a malignant human melanoma cell line that displays aneuploid fibroblastic morphology and grows in adherent tissue culture. This cell line has been reported to be nonpermissive for human cytomegalovirus (HCMV). HT-144 cells form xenograft tumors when injected into immunocompromised mice. These cells contain a mutation in the ATM gene, resulting in the expression of a truncated protein, which causes increased sensitivity to UVB and ionizing radiation compared to other melanoma cell lines. The HT-144 cells also express mutant B-Raf (V600E).

Source

This cell line was established in 1966 from a metastatic site (subcutaneous tissue) in a 29-year-old Caucasian male with malignant melanoma.

Inventors

  • Jorgen Fogh, PhD, formerly at Sloan Kettering Institute, Memorial Sloan Kettering
  • Germaine Trempe, formerly at Sloan Kettering Institute, Memorial Sloan Kettering

Key References

  • Fogh J et al. (1977) One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. Journal of the National Cancer Institute 59: 221-226 (PubMed ID: 327080)
  • Smith JD (1986) Human cytomegalovirus: demonstration of permissive epithelial cells and nonpermissive fibroblastic cells in a survey of human cell lines. Journal of Virology 60: 583-588 (PubMed ID: 3021992)
  • Ramsay J et al. (1998) Radiosensitive melanoma cell line with mutation of the gene for ataxia telangiectasia. British Journal of Cancer 77: 11-14 (PubMed ID: 9459139)
  • Chen B et al. (2012) BRAFV600E negatively regulates the AKT pathway in melanoma cell lines. PLoS One 7: e42598 (PubMed ID: 22880048)

Licensing Information

This cell line may be licensed nonexclusively for research or commercial purposes.

Contact Information

  • For licensing requests: Alexandra Buga, MBA, Licensing Associate, Office of Technology Development, MSK, 646-888-1078, bugaa@mskcc.org
  • For non-licensing requests from academic-research institutions: Frances Weis-Garcia, PhD, Associate Laboratory Member/Head, Antibody & Bioresource Core Facility, MSK, 646-888-2354, weisgarf@mskcc.org

Stage of Development

Ready to use

Indications

Cancer › Melanoma

Types

Research Tools › Cell Lines