IGO performs extraction of RNA and DNA from both standard and challenging sources, followed by sample quality assessment. Sample preparation may also include pathological review, phenotypic characterization, and microdissection from FFPE or OCT preserved tissues.
After an internal quality assessment, we provide feedback and recommendations as to which downstream platform is most practical. These recommendations are based off the performance of thousands of past samples and are meant to allow researchers to make the most informed decision possible.
IGO prepares Illumina libraries for diverse Next-generation sequencing (NGS) applications. While we have optimized our standard preparation to be run on an automated platform, manual preparation is employed for technically challenging samples.
Library preparation services currently offered by IGO:
Whole genome sequencing
- Human/mouse (deep and shallow)
- Shotgun metagenomics
Whole transcriptome sequencing
- Poly A enrichment
- SMART-Seq for ultra-low input
- CRISPR Screens
- 16S sequencing
Targeted sequencing (for full gene lists, please contact us at [email protected])
- MSK-IMPACT (mouse and human)
- MSK ACCESS
- Whole exome sequencing (mouse and human)
- Custom targeted panels (contact [email protected] for more information)
- IGO in-house platform
- 10x genomics VDJ
- CRISPR cut site sequencing
- Archer FusionPlex assay
Single Cell & Spatial Transcriptomics
IGO maintains a full complement of technologies to interrogate gene expression, immune repertoire, protein expression, chromatin accessibility, and DNA-based SNVs/CNVs of single cells. We also offer frozen- and FFPE-based high resolution spatial transcriptomics platforms to examine expression in context of a tissue or tumor.
10X Genomics scRNA-Seq
- 3’ or 5’ GEX
- Optional VDJ enrichment
- Optional feature barcoding or cell hashing
10X Genomics Multiome
- Single nuclei ATAC-Seq + RNA-Seq
- 10X Genomics Visium
- MissionBio Tapestri
- Nanostring GeoMx
- SMART-Seq 2 method for low cell numbers
- Direct Library Prep
IGO houses a full range of Illumina sequencing instruments including MiSeq, NextSeq 500 and 2000, and NovaSeq 6000, allowing researchers to choose from a variety of read lengths and depths.
Before sequencing, users must consider how many reads are required (read depth) and how long the reads should be (read length). We encourage users to discuss their experimental design with their bioinformatician prior to starting an experiment and collecting samples. MSK’s Bioinformatics Core Facility (BIC) can help you define these values, if you’re unsure of what sequencing parameters are required to answer your biological questions. You can contact them at [email protected].
IGO also offers full-lane sequencing for users who prepare their own libraries. Lane sizes range from 15 million reads (MiSeq) to multiples of 400M reads (Next/NovaSeq) We can assist with planning and pooling of libraries and make suggestions to ensure quality data output. Please contact the core at any time with your user-made libraries for sequencing so that we can ensure the library is compatible with Illumina sequencers.
IGO has recently acquired the MinION and PromethION. Contact [email protected] for more details and pricing.
In addition to NGS, IGO also offers custom fragment analysis and Cell Line Authentication by STR analysis. Fragment analysis on the ABI SeqStudio is ideal for allelic detection and identification and microsatellite analysis, while STR is commonly used for human cell line authentication and T/N matching confirmation.
As an alternative to real-time quantitative PCR, IGO provides digital PCR (dPCR) services for mutation detection, fusion detection, viral detection or CNV. IGO has already verified many genes, and the full list can be requested by e-mailing [email protected].
If your gene of interest has not been verified, IGO can perform new assay design and optimization.