Saos-2 is a human osteosarcoma cell line, which displays several osteoblastic features. These cells express receptors for 1,25-dihydroxyvitamin D3 and have high basal alkaline-phosphatase activity. They express the parathyroid hormone (PTH) receptor and produce cyclic AMP in response to treatment with PTH. These cells do not form tumors when injected subcutaneously into immunocompromised mice. When injected into diffusion chambers that are implanted intra-peritoneally into immunocompromised mice, however, Saos-2 cells produce a mineralized matrix, which is a defining characteristic of osteoblastic cells. All of these characteristics make this cell line an attractive source of bone-related molecules for research.
This cell line was established in 1973 from an 11-year-old Caucasian female with osteogenic sarcoma.
Jorgen Fogh, PhD, formerly at Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center
- Fogh J et al. (1977) Absence of HeLa cell contamination in 169 cell lines derived from human tumors. Journal of the National Cancer Institute 58: 209-214 (PubMed ID: 833871)
- Rodan SB et al. (1987) Characterization of a human osteosarcoma cell line (Saos-2) with osteoblastic properties. Cancer Research 47: 4961-4966 (PubMed ID: 3040234)
This cell line may be licensed nonexclusively for research or commercial purposes.
For internal research purposes by a commercial entity: Utilize MSK’s Express License, attached here. In order to streamline and expedite the licensing of select MSK cell lines, the terms of this non-exclusive license are non-negotiable. Please note: This license is only available for select cell lines, including this one.
To proceed, please download this contract, fill in all relevant fields, and email a scanned version to [email protected]. Once this license has been executed by MSK, you will receive a signed copy and invoice, and can then place your order with ATCC.
For licensing requests for a commercial purpose: Contact MSK’s Tangible Materials team at [email protected].
For non-licensing requests from academic-research institutions: Contact Frances Weis-Garcia, PhD, Associate Laboratory Member/Head, Antibody & Bioresource Core Facility, MSK, 646-888-2354, [email protected].