Tumor-Associated Extracellular Vesical and Particles (EVPs) in Early-Stage Lung Cancer

The David Jones Lab
Projects
Publications

Tumor-Associated Extracellular Vesical and Particles (EVPs) in Early-Stage Lung Cancer

Share
Share

Exosomes are 30–150 nm nanovesicles of endosomal origin that mediate intercellular communication in normal physiology and pathology. Tumor-derived exosome proteins or extracellular vesical and particles (EVPs) have prognostic and functional importance in tumor progression, immune regulation, and metastasis. Working collaboratively with the David Lyden Laboratory at Cornell, we examine the role of tumor-associated exosomes in lung adenocarcinoma (LUAD) and their ability to influence the tumor microenvironment and the biology of metastases. We recently opened a prospective clinical trial examining EVPs in patients with clinicopathologic-annotated LUAD before and after surgical resection of their primary tumor using blood, cancer, and normal tissue with a specific interest in exosomal proteomics, miRMA, and DNA. We believe this project will allow us to better understand the role of EVPs in the biology of lung cancer metastases, as well as potentially using EVPs as a biomarker for tumor aggressiveness and response to treatment.

  1. Rodrigues G, Hoshino A, Matei IR, Scandariato I, Kenific CM, Kim HS, Casanova-Salas I, Dai D, Badwe CR, Gril B, Mark MT, Dill BD, Molina H, Benito-Martin A, Bojmar L, Offer K, LaPlant Q, Ararso Y, Buehring W, Wang H, Jiang X, Liu Y, Sabari JK, Shin SJ, Narula N, Rajasekhar VK, Zhang H, Costa Silva B, Rafii S, Rudin CM, Jones DR, Steeg PS, Peinado H, Ghajar CM, Bromberg J, Pisapia D, de Sousa M, Lyden D. Tumour exosomal KIAA1199 protein promotes cancer cell colonization in brain metastasis. Nat Cell Biol 2019;21:1403-12.
  2. Hoshino A, Kim HS, Bojmar L, Gyan KE, Hernandez J, Zambirinis C, Rodrigues G, Mark MT, Molina H, Di Giannatale A, Offer K, Nakajima M, Martin AB, Williams C, Nogués L, Vatter FA, Hashimoto A, Alexander E. Davies AE, Freitas D, Steiner L, Ararso Y, Buehring W, Ogitani Y, Aleckovic M, Wang H, Schaeffer M, Balachandran VP, Karajannis M, Dunkel I, Gilheeney S, Khakoo Y, Raju P, Scherz A, Sagi I, Scherz-Shouval R, Yarden Y, Petriccione M, De Braganca K, Donzelli M, Fischer C, Vitolano S, Wright G, Ganshaw L, Marrano M, Ahmed A, DeStefano J, Danzer E, Rohr M, Vincent T, Weiser MR, Brady MS, Meyers P, Wexler LH, Ambati S, Cheung A, Slotkin E, Modak S, Roberts S, Cheung NK, Basu E, Kushner B, Diolaiti D, Krantz B, Simpson AL, Berger M, Rudin CM, Simeone D, Ghajar C, Batra SK, Stanger BZ, Bui J, Brown KA, Rajasekhar VK, Healey JH, de Sousa M, Kelsen D, Kramer K, Sheth S, Baisch J, Pascual V, Heaton TE, La Quaglia MP, Pisapia DJ, Schwartz R, Zhang H, Liu Y, Shukla A, Bissell M, Grandgenett P, Hollingsworth M, Bromberg J, Costa-Silva B, Peinado H, Kang Y, Garcia BA, O’Reilly E, Trippett TM, Jones DR, Matei I, Jarnagin WR, Lyden D. Human-derived exosome proteins define tumor-associated biomarkers and potential therapeutic targets for multiple cancers. Cell 2020;182:1044-61.
  3. Bojmar L, Kim HS, Tobias GC, Pelissier Vatter FA, Lucotti S, Gyan KE, Kenific CM, Wan Z, Kim KA, Kim D, Hernandez J, Pascual V, Heaton TE, La Quaglia MP, Kelsen D, Trippett TM, Jones DR, Jarnagin WR, Matei IR, Zhang H, Hoshino A, Lyden D. Extracellular vesicle and particle isolation from human and murine cell lines, tissues, and bodily fluids. STAR Protoc 2020;2(1):100225.