BRMS1-Mediated Suppression of Metastases in Lung Adenocarcinoma


In LUAD, p53 mutations occur more commonly (45%) than KRAS, EGFR, or ELM4/ALK genomic alterations, combined. Previously, we have shown that BRMS1-mediated suppression of cell migration and invasion is associated with loss of p53. An unbiased analysis of the TCGA database reveals low BRMS1 expression is associated with more lymphovascular invasion and worse progression-free survival in p53mut LUAD and LUAD with solid histologic subtype. Our lab has identified that BRMS1 suppresses metastasis through multiprotein HDAC transcriptional co-repressor complex or as an E3 ligase to control p300 acetyltransferase levels. We have also shown that BRMS1 is repressed both at the transcriptional level by DNMT1 and post-translationally by CK2-induced phosphorylation and proteosome degradation (Figure 1). Using a CK2 inhibitor (CX4945), we have been able to rescue the expression of BRMS1 and repress metastases, showing BRMS1 may be therapeutically targetable.  We have also found that the homozygous SNP rs1052566 in BRMS1v2 (BRMS1v2A273V/A273V) is present in 8% of patients with LUAD and correlates with tumor aggressiveness. We discovered that inactivation of nuclear Src mediates the accumulation of intratumoral c-fos in patients with BRMS1v2A273V/A273V LUAD, which promotes metastases. Using a patient-derived organoid (PDO) metastasis model, we utilized a c-fos inhibitor to suppress metastases in BRMS1v2A273V/A273V LUAD (Figure 2). To better characterize mechanisms of action and functional assessments in vivo, we have also generated the first Brms1-/- transgenic mouse by crossbreeding GEMM and a mouse cell line, obtaining transgenic mice with oncogenic KRAS+/LSL-G12D/ p53fl/fl with different Brms1 profiles (Brms1-/-, +/+, +/-) (KPB mice) (Figure 3). We continue to use our PDO model and novel BRMS1-/- transgenic mice to further understand the transcriptional mechanisms of BRMS1-mediated suppression of metastases in p53mut LUAD. We collaborate with the Marty W. Mayo Laboratory, the Jorge Reis-Filho Laboratory, and the Prasad S. Adusumilli Laboratory.

BMSR1 Models

Figure 1. CK2a’-induced BRMS1 degradation promotes metastasis of NSCLC in vivo. A. Induction of CK2a’ promotes distant metastasis of NSCLC. H157 stable cell lines were orthotopically injected into the left lung of mice. Mouse bioluminescent CT images show the growth of tumor and metastatic sites in three representative mice in each group (1–3, control; 4–6, BRMS1WT without doxycycline; 7–9, BRMS1WT with doxycycline (Doxy.); 10–12, BRMS1S30A with doxycycline). The scale indicates the signal intensity cell quantification. B. Activation of CK2a’ induces metastasis of tumors with BRMS1 wild-type. The graph represents fold changes of metastasis burden over control group 43 days post-injection. (Cancer Res 2016)

Figure 2

Figure 2. Schematic illustration of putative mechanism of BRMS1v2 A273V-promoted LUAD metastases. BRMS1v2 WT interacts with Src which induces tyrosine phosphorylation, nuclear exportation and degradation of c-fos and inhibits CEACAM6 transcription and metastases. In contrast, BRMS1v2 A273V is unable to bind and active Src, resulting in upregulation of c-fos-induced transcription of CEACAM6 which promotes metastases

Figure 3

Figure 3. Generation of Brms1-/-  transgenic mice. A. Brms1 copy numbers by Taqman assays. B. Brms1 mRNA (Red) and DAPI (Blue) on the lungs by Fish.


  1. Liu Y, Chudgar N, Mastrogiacomo B, He D, Lankadasari MB, Bapat S, Jones GD, Sanchez-Vega F, Tan KS, Schultz N, Mukherjee S, Offit K, Bao Y, Bott MJ, Rekhtman N, Adusumilli PS, Li BT, Mayo MW, Jones DR. A germline SNP in BRMS1 predisposes patients with lung adenocarcinoma to metastasis and can be ameliorated by targeting c-fos. Sci Transl Med. 2022;14(665):eabo1050.
  2. Liu Y, Amin EB, Mayo MW, Chudgar NP, Bucciarelli PR, Kadota K, Adusumilli PS, Jones DR. CK2α’ Drives Lung Cancer Metastasis by Targeting BRMS1 Nuclear Export and Degradation. Cancer Res 2016;76(9):2675-2686.