Mouse Hospital Unit

Graphic with Mouse Hospital in a box in the middle with arrows pointing out to eight different services/functions written in boxes

The mouse hospital supports the translational integration of mouse and human trials at MSK.

The Mouse Hospital Unit was established to strengthen infrastructural needs for the translational integration of mouse and human clinical trials at MSK. This unit ensures that mouse trials effectively mimic treatment plans of human patients, especially with regard to treatment schedule, clinical observations and outcome assessment.


Toxicology and Pharmacology:

  • MTD, acute and chronic toxicity studies in rodents
  • PK/PD assessment of agents in mice, rats, dogs


  • Evaluation of efficacy of therapeutic agents in relevant mouse models
  • Best route/dose/administration schedule

Technical Assistance:

  • Highly specialized assistance on all aspects of in vivo studies


  • Design of in vivo studies
  • Assistance with IACUC, IRB, IND applications and grants writing

Toxicity Studies

Formulation and delivery: Compounds are formulated for in vivo delivery using a variety of commonly used vehicles, and are administered by a number of routes including: oral gavage (p.o.), intravenous (i.v.), intraperitoneal (i.p.), subcutaneous (s.c.), intratumor (i.t.) and by food and/or drinking water. Additionally, Alzet osmotic minipumps are implanted for continuous drug delivery, and slow release hormones pellets are implanted s.c.

Exploratory rodent toxicology studies: We support studies to determine Maximum Tolerated Dose (MTD) after single-dose or chronic exposure required for tolerance profiling of candidate agents.

Rodent safety toxicology studies: We perform acute single-dose or repeat-dose toxicity studies for different test articles (small, large molecules, biologics, stem cells) in mice in support of IND applications. These are performed in full compliance with Good Laboratory Practice (GLP) regulations, and can be presented in SEND compliant format. We coordinate our efforts with MSK IND/IDE Development Office, so that GLP Safety Toxicology Reports are incorporated into the Pharm/Tox section of MSK- sponsored IND applications.

PK/PD Studies

Upon compound administration (in mice, rats and dogs), plasma, organs and tumor samples are collected at multiple times to assess bioavailability and pharmacokinetic (PK), Pharmacodynamic (PD) and Toxicokinetic (TK) profile of therapeutic agents.

Vanessa Thompson, PhD
Elisa de Stanchina, PhD

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Subject line: Toxicology service request

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Subject line: Pharmacology services request

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Antitumor Facility

Mark Satter
IP Quality Assurance Manager

Sonia Sequeira
IND/IDE Development ManagerEfficacy Studies

We evaluate in vivo efficacy of agents either alone or in combination with standard chemotherapy regimens against murine tumors, human tumor xenografts and transgenic mouse models of human cancer. Tumor size is monitored by caliper measurement (for s.c. xenografts) and/or in vivo imaging (for disseminated and orthotopic models).

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Send email with Subject: Efficacy Study request to:
Elisa de Stanchina

(left) Four nude mice superimposed on line graph and (right) MRIs of tumors in mice

Subcutaneous xenografts and in vivo imaging allow for tumor size measurement to evaluate efficacy of agents.

We provide technical assistance on all aspects of in vivo studies including: rodent surgeries, tumor cell implantation, tumor transplantation, bone marrow transplants, drug formulation and administration (intravenous, intraperitoneal, oral, intratumor, intradermal, subcutaneous), radiation therapy, x-ray, IVIS, MRI and PET imaging, hematology and clinical chemistry analyses, collection of fluids (blood, urine), collection and preservation of tissues/organs for either RNA/protein or HIC analysis.

  • Rodent surgeries
  • Blood collection
  • Tissues collection and processing
  • Bone marrow transplants
  • Radiation therapy
  • In vivo imaging
  • Drug formulation and administration

List of all services provided:


Bone (intra femur)
Brain (jntracranial)
Breast (Mammary fat pad and intraductal)
Lung (Intrapleural)
Subcutaneous and intradermal


Intravenous (bolus)
Intravenous (Infusion)
Intravenous (high pressure)
Retro orbital
Sub cutaneous
Oral (gavage)
Cardiac perfusion
Implantation of infusion pumps via jugular catheter
Implantation of Alzet minipumps
Implantation of estrogen/testosterone pellets s.c.


Sub cutaneous tumor resection
Mammary Fat Pad Tumor resection


Blood collection (tail, cheek, retro-orbital, cardiac puncture)
Blood analysis (Hematology and clinical chemistry)
Bone Marrow Aspirates
Tumor/organs harvesting and processing
Necropsy and organ dissection services
Urine collection
Ear punching
Ear tagging
Tail Tattoo
Breeding (for specific projects only)
Cell culture (for xenograft studies)
Single cell suspension from Tumor samples (Gentlemacs)
Imaging (IVIS) – Bioluminescence and Fluorescence
Irradiation (whole body and X-Ray targeted radiation)


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Send email with SUBJECT: Technical Assistance request to:
Elisa de Stanchina

We provide consulting services to investigators in designing and planning their preclinical studies and in analyzing and summarizing data for publications and IND applications. Additionally, we assist investigators in writing animal protocols and grant applications involving animal studies. We also typically act as a central coordinator so that studies involving support from several core facilities are carried out properly and in a timely and cost-efficient way.

  • Study design (MTD, Efficacy, Safety Toxicology, PK/PD)
  • Experimental data analysis
  • Biostatistics support
  • Writing of IRB and IACUC protocols
  • Writing of IND- Enabling Toxicology studies
  • Writing of SOPs
  • Other aspects of IND Application (CMC and clinical protocols)
  • Good Laboratory Practice (GLP) compliance
  • Help with grant writing: in vivo studies design, animal number justification (VAS), IACUC, IBC and IRB compliance

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