More About Center for Precision Disease Modeling Minus iconIcon indicating subtraction, or that the element can be closed. Plus IconIcon indicating addition, or that the element can be opened. Arrow (down) icon.An arrow icon, usually indicating that the containing element can be opened and closed.

Mouse Hospital Unit

Graphic with Mouse Hospital in a box in the middle with arrows pointing out to eight different services/functions written in boxes

The Mouse Hospital Unit was established to strengthen infrastructural needs for the translational integration of mouse and human clinical trials at MSK. This unit ensures that mouse trials effectively mimic treatment plans of human patients, especially with regard to treatment schedule, clinical observations and outcome assessment.

Services

Toxicology and Pharmacology:

  • MTD, acute and chronic toxicity studies in rodents
  • PK/PD assessment of agents in mice, rats, dogs

Efficacy:

  • Evaluation of efficacy of therapeutic agents in relevant mouse models
  • Best route/dose/administration schedule

Technical Assistance:

  • Highly specialized assistance on all aspects of in vivo studies

Consulting:

  • Design of in vivo studies
  • Assistance with IACUC, IRB, IND applications and grants writing
Toxicity and Pharmacology Minus iconIcon indicating subtraction, or that the element can be closed. Plus IconIcon indicating addition, or that the element can be opened. Arrow (down) icon.An arrow icon, usually indicating that the containing element can be opened and closed.

Toxicity Studies

Formulation and delivery: Compounds are formulated for in vivo delivery using a variety of commonly used vehicles, and are administered by a number of routes including: oral gavage (p.o.), intravenous (i.v.), intraperitoneal (i.p.), subcutaneous (s.c.), intratumor (i.t.) and by food and/or drinking water. Additionally, Alzet osmotic minipumps are implanted for continuous drug delivery, and slow release hormones pellets are implanted s.c.

Exploratory rodent toxicology studies: We support studies to determine Maximum Tolerated Dose (MTD) after single-dose or chronic exposure required for tolerance profiling of candidate agents.

Rodent safety toxicology studies: We perform acute single-dose or repeat-dose toxicity studies for different test articles (small, large molecules, biologics, stem cells) in mice in support of IND applications. These are performed in full compliance with Good Laboratory Practice (GLP) regulations, and can be presented in SEND compliant format. We coordinate our efforts with MSK IND/IDE Development Office, so that GLP Safety Toxicology Reports are incorporated into the Pharm/Tox section of MSK- sponsored IND applications.

PK/PD Studies

Upon compound administration (in mice, rats and dogs), plasma, organs and tumor samples are collected at multiple times to assess bioavailability and pharmacokinetic (PK), Pharmacodynamic (PD) and Toxicokinetic (TK) profile of therapeutic agents.

Staff:
Veron Browne, PhD
Elisa de Stanchina, PhD
Vanessa Thompson, PhD

Request service:

Subject line: Toxicology service request

Send email to:
brownev@mskcc.org
destance@mskcc.org

Subject line: Pharmacology services request

Send email to:
thompsv1@mskcc.org
destance@mskcc.org

Access our websites:
Antitumor Facility

Mark Satter
IP Quality Assurance Manager

Sonia Sequeira
IND/IDE Development ManagerEfficacy Studies

Efficacy Studies Minus iconIcon indicating subtraction, or that the element can be closed. Plus IconIcon indicating addition, or that the element can be opened. Arrow (down) icon.An arrow icon, usually indicating that the containing element can be opened and closed.

We evaluate in vivo efficacy of agents either alone or in combination with standard chemotherapy regimens against murine tumors, human tumor xenografts and transgenic mouse models of human cancer. Tumor size is monitored by caliper measurement (for s.c. xenografts) and/or in vivo imaging (for disseminated and orthotopic models).

Access our LabPortal

Request service:
Send email with Subject: Efficacy Study request to:
Elisa de Stanchina
destance@mskcc.org

(left) Four nude mice superimposed on line graph and (right) MRIs of tumors in mice
Technical Assistance Minus iconIcon indicating subtraction, or that the element can be closed. Plus IconIcon indicating addition, or that the element can be opened. Arrow (down) icon.An arrow icon, usually indicating that the containing element can be opened and closed.

We provide technical assistance on all aspects of in vivo studies including: rodent surgeries, tumor cell implantation, tumor transplantation, bone marrow transplants, drug formulation and administration (intravenous, intraperitoneal, oral, intratumor, intradermal, subcutaneous), radiation therapy, x-ray, IVIS, MRI and PET imaging, hematology and clinical chemistry analyses, collection of fluids (blood, urine), collection and preservation of tissues/organs for either RNA/protein or HIC analysis.

  • Rodent surgeries
  • Blood collection
  • Tissues collection and processing
  • Bone marrow transplants
  • Radiation therapy
  • In vivo imaging
  • Drug formulation and administration

List of all services provided:

 

CELL LINE AND PDX ORTHOTOPIC IMPLANTATIONS
Bladder
Bone (intra femur)
Brain (jntracranial)
Breast (Mammary fat pad and intraductal)
Colon
Kidney
Liver
Lung (Intrapleural)
Muscle
Pancreas
Prostate
Ovary
Spleen
Stomach
Subcutaneous and intradermal
Thyroid
Tongue
Uterus

 

INJECTIONS and DOSING
Intravenous (bolus)
Intravenous (Infusion)
Intravenous (high pressure)
Intraperitoneum
Retro orbital
Intracardiac
Intramuscle
Sub cutaneous
Intratumor
Oral (gavage)
Cardiac perfusion
Implantation of infusion pumps via jugular catheter
Implantation of Alzet minipumps
Implantation of estrogen/testosterone pellets s.c.

 

RESECTIONS and OTHER SURGERIES
Sub cutaneous tumor resection
Mammary Fat Pad Tumor resection
Castration
Nephrectomy
Ovariectomy
Splenectomy

 

OTHER PROCEDURES
Blood collection (tail, cheek, retro-orbital, cardiac puncture)
Blood analysis (Hematology and clinical chemistry)
Bone Marrow Aspirates
Tumor/organs harvesting and processing
Necropsy and organ dissection services
Urine collection
Ear punching
Ear tagging
Tail Tattoo
Breeding (for specific projects only)
Cell culture (for xenograft studies)
Single cell suspension from Tumor samples (Gentlemacs)
Imaging (IVIS) – Bioluminescence and Fluorescence
Irradiation (whole body and X-Ray targeted radiation)

 

Request service:
Send email with SUBJECT: Technical Assistance request to:
Elisa de Stanchina
destance@mskcc.org

Consultation Services Minus iconIcon indicating subtraction, or that the element can be closed. Plus IconIcon indicating addition, or that the element can be opened. Arrow (down) icon.An arrow icon, usually indicating that the containing element can be opened and closed.

We provide consulting services to investigators in designing and planning their preclinical studies and in analyzing and summarizing data for publications and IND applications. Additionally, we assist investigators in writing animal protocols and grant applications involving animal studies. We also typically act as a central coordinator so that studies involving support from several core facilities are carried out properly and in a timely and cost-efficient way.

  • Study design (MTD, Efficacy, Safety Toxicology, PK/PD)
  • Experimental data analysis
  • Biostatistics support
  • Writing of IRB and IACUC protocols
  • Writing of IND- Enabling Toxicology studies
  • Writing of SOPs
  • Other aspects of IND Application (CMC and clinical protocols)
  • Good Laboratory Practice (GLP) compliance
  • Help with grant writing: in vivo studies design, animal number justification (VAS), IACUC, IBC and IRB compliance

Request service:

Subject line: Consultation service request

Send email to:
destance@mskcc.org