Clone TA99 is a mouse monoclonal antibody that reacts with tyrosinase-related protein 1 (TRP1), a 75kDa differentiation-related human glycoprotein (gp75), formerly referred to as pigmentation-associated antigen (PAA). It is expressed by pigmented melanoma cells and cultured melanocytes. TRP1 is involved in the pigmentation machinery of melanocytes and can be used as a differentiation marker.
This antibody was derived in 1985 by injection of whole human melanoma cells (SK-MEL-23) into mice. Splenocytes from these immunized mice were fused with NS-1 cells to generate hybridomas producing anti-TRP1 antibodies.
- Francisco X. Real, MD, PhD, formerly at Memorial Sloan Kettering
- Lloyd J. Old, MD, former William E. Snee Chair in Cancer Immunology, Memorial Sloan Kettering; former Director, New York Branch, Ludwig Institute for Cancer Research
- Timothy M. Thomson, MD, PhD
- Thomson TM et al. (1985) Pigmentation-Associated Glycoprotein of Human Melanomas and Melanocytes: Definition with a Mouse Monoclonal Antibody. Journal of Investigative Dermatology 85: 169-174 (PubMed ID: 3926906)
- Bevaart L et al. (2006) The high-affinity IgG receptor, FcgammaRI, plays a central role in antibody therapy of experimental melanoma. Cancer Research 66: 1261-1264 (Pubmed ID: 16452176)
This hybridoma may be licensed nonexclusively for commercial purposes. For more information, please contact TRMOTDRTM@mskcc.org.
Purified antibody may be available for sale through the Memorial Sloan Kettering Antibody & Bioresource Core Facility. Please email firstname.lastname@example.org for further information.