The Molecular Epidemiology Laboratory

Led by Dr. Irene Orlow, the Molecular Epidemiology Laboratory participates in self-initiated research & supports investigations from the Population Sciences Research and Clinical Research programs that involve handling and testing of a variety of human specimens.

How we do it

Experimental Approach

• Aims aligned with type of biospecimens, and short- and long-term analytical plan
• Analytical plan determines initial processing
• Sample processing plan is reviewed and technology is selected
• Assays are designed and newly developed as needed, and validated
• Quality assurance (QA) and quality control (QC) delineated

Biospecimens & Logistics

• Collection details and labeling reviewed
• Kits and instructions provided
• Delivery details and communications instructed
• Biosafety considered at each step

Biospecimens Handling & Assay Development

• Appropriate range of laboratory techniques
• Step-by step environment considerations
• Isolation
• Quality- , quantity- ,  and viability- assessment
• Empirical assay design

Testing & Data Generation

• Validation of assay, selection of lab controls
• Tracking and deliverables established
• Testing of Study Specimens and laboratory controls

Quality Control & Data Handling

• Review of visual data
• Data entry
• Descriptive analysis, and comparison to empirical parameters
• Data cleaning and summary

Samples Distribution & Banking

• Establishment of sample volumes and configuration or layout
• Selection of the most appropriate environmental conditions
• Aliquoting for collaborating laboratories and consortia
• Preliminary preparation for high throughput testing
• Banking for additional and/or future investigations
• Monitoring on-site, off-site, and QC