More about 5hmC-seq
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While EM-seq allows the detection of both the 5mC and 5hmC DNA modifications undistinguishably, 5hmC-seq is a new method for the specific detection of the 5hmC DNA modification at the single base level.
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As with EM-seq, Enzymatic 5hmC-seq (E5hmC-seq) uses an enzyme-based method to specifically detect 5hmC, without the DNA damage typical of bisulfite-based methods, and this sensitive method can be used with as little as 0.1 ng of input DNA.
References:
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Pastor, W.A., et al., Genome-wide mapping of 5-hydroxymethylcytosine in embryonic stem cells. Nature, 2011. 473(7347):394-7 Pastor, W.A. et al.
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Sun et al., Nondestructive enzymatic deamination enables single-molecule long-read amplicon sequencing for the determination of 5-methylcytosine and 5-hydroxymethylcytosine at single-base resolution. Genome Research, 2021. 31(2):291-300 Sun Z et al.