another enzyme-tethering strategy to profile genomic binding sites for the protein of interest. In CUT&Tag, a target protein is bound in situ by a specific antibody, which then tethers a protein A-Tn5 transposase fusion protein. Activation of pA-Tn5 results in factor-targeted tagmentation, generating fragments ready for PCR enrichment and DNA sequencing. CUT&Tag has high resolution and exceptionally low background using low cell numbers and even single cells. The entire procedure from live cells to sequencing-ready libraries can be performed in one day.
Reference: Kaya-Okur, H.S., et al., CUT&Tag for efficient epigenomic profiling of small samples and single cells. Nat Commun, 2019. 10(1): p. 1930.