More about Nanopore Sequencing


Nanopore Sequencing:
a third generation sequencing technology that enables direct, real-time analysis of long DNA or RNA fragments. It works by monitoring changes to an electrical current as nucleic acids are passed through a protein nanopore. The resulting signal is decoded to provide the specific DNA or RNA sequence along with the identification of base modifications. Using nanopore sequencing, a single molecule of DNA or RNA can be sequenced without the need for PCR amplification or chemical labeling of the sample.


1) Clarke, J., et al., Continuous base identification for single-molecule nanopore DNA sequencing. Nat Nanotechnol, 2009. 4(4): p. 265-70.

2) Soneson, C., et al., A comprehensive examination of Nanopore native RNA sequencing for characterization of complex transcriptomes. Nat Commun, 2019. 10(1): p. 3359.